中国医科大学学报2017，Vol.46Issue(3)：201-204,209,5.DOI:10.12007/j.issn.0258-4646.2017.03.003
miR-29s家族对人脑胶质瘤干细胞生物学行为的影响
Effects of miR-29s Family on the Biological Behaviors of Glioma Stem Cells
摘要
Abstract
Objective To investigate the expression of miR-29s in the glioma stem cells,and explore how the members of miR-29s affect the bio-logical behaviors of glioma stem cells. Methods Eight patient specimens were used to culture glioma stem cells. Real-time PCR was adopted to test the expression of miR-29s. CCK-8 analysis was performed to test the proliferation ,Transwell was used to test cell migration and invasion ,and flow-cytometry analysis was carried out to test apoptosis. Results miR-29a,miR-29b and miR-29c were decreased in glioma stem cells. Over-ex-pression of miR-29s could inhibit the proliferation,cell migration and invasion,but promote apoptosis of glioma stem cells. Conclusion miR-29s acts as a cancer suppressor gene in the glioma stem cells ,and miR-29a plays the dominant functional role in the family.关键词
胶质瘤干细胞/miR-29a/miR-29b/miR-29c/生物学行为Key words
glioma stem cells/miR-29a/miR-29b/miR-29c/biological behaviors分类
医药卫生引用本文复制引用
奚卓,薛一雪,马珺,刘云会..miR-29s家族对人脑胶质瘤干细胞生物学行为的影响[J].中国医科大学学报,2017,46(3):201-204,209,5.基金项目
国家自然科学基金(81672511,81573010) （81672511,81573010）
辽宁省科学技术计划(2015225007) （2015225007）
中国医科大学附属盛京医院自由研究者计划(201304) （201304）
