中国医科大学学报2017，Vol.46Issue(3)：210-213,218,5.DOI:10.12007/j.issn.0258-4646.2017.03.005
茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响
Effects of Epigallocatechin-3-gallate on Oxidative Stress and Inflammatory Factors in the Kidney Tissues of Rats with Paraquat Poisoning
摘要
Abstract
Objective To investigate the effect of Epigallocatechin-3-gallate(EGCG)on oxidative stress and inflammatory factors in the kidney tissues of rats with paraquat poisoning,and explore the possible mechanism. Methods Rats were randomly divided into control group(group A), paraquat exposure group(group B),paraquat exposure+low dose of EGCG group(group C),and paraquat exposure+high dose of EGCG group (group D)(n=10 in each group). Kidney tissues were harvested and observed by HE staining. The level of creatinine was tested by biochemical detector. Serum oxidative stress and inflammatory factor level were detected by ELISA method. Oxidative stress in kidney tissue was determined by Western blotting and colorimetry. Inflammatory factor level in renal tissue was tested by real-time PCR. Results Kidney damage was observed in rats of groups B,C,and D. Rats in groups C and D showed less renal injury than group B,and high dose of EGCG(group D)enhanced kidney damage compared to the low dose(group C). Compared with group A,rats in the groups C and D showed lower level of 8-isoprostane,creatinine, interleukin-6(IL-6),and tumor necrosis factorα(TNF-α)in serum and malondialdehyde(MDA),3-nitrotyrosine(3-NT),and TNF-αand IL-6 mRNA in renal tissue in rats with paraquat poisoning,and group D showed the lowest mRNA level of inflammatory factor and oxidative stress. Con-clusion EGCG has protective effects on the kidney of rats with paraquat poisoning,and its mechanism may be related to the reduction of oxida-tive stress and inflammatory reaction in the kidney.关键词
茶多酚/百草枯/氧化应激/炎症反应/肾脏Key words
epigallocatechin-3-gallate/paraquat/oxidative stress/inflammatory reaction/kidney分类
医药卫生引用本文复制引用
沈海涛,吴娜,赵宏宇,胡晓,郭峰,赵敏..茶多酚对百草枯中毒大鼠肾脏氧化应激及炎症反应的影响[J].中国医科大学学报,2017,46(3):210-213,218,5.基金项目
卫生部国家临床重点专科建设项目(2012-649) （2012-649）
