中国医科大学学报2017，Vol.46Issue(3)：223-226,237,5.DOI:10.12007/j.issn.0258-4646.2017.03.008
改良脊髓半横断损伤大鼠模型的建立及评价
Establishment and Evaluation of Improved Hemisection Model of Spinal Cord Injury in Rats
摘要
Abstract
Objective To establish an improved hemisection model of spinal cord injury(SCI)in rats with less severe injury and increased sur-vival rate. Methods Female Sprague-Dawley rats(n=20)were randomly divided into 2 groups:standard and improved hemisection model of SCI. Basso-Beattie-Bresnahan(BBB)scores were carried out to determine the hindlimb locomotor function at 1 d,3 d,1 week and 2 weeks post SCI,respectively. In addition,duration of operation,blood loss,location of posterior midline,and success rate of spinal cord exposure during the second surgery were used as the main parameters to evaluate the significance of the new model. Results Compared with the standard hemisection model,the improved hemisection model of SCI exhibited shorter duration of operation,less blood loss,higher BBB score,more accurate location of posterior midline,and higher success rate of spinal cord exposure during the second surgery. Based on the improved hemisection model of SCI, transplantation model of scar tissue in spine cord was then successfully constructed with a success rate above 70%. Conclusion The improved hemisection model of SCI shows significant advantages in many aspects related to the operation,which is applicable to the study of SCI,especially the non-acute SCI.关键词
脊髓损伤/大鼠模型/改良Key words
spinal cord injury/rat models/modification分类
医药卫生引用本文复制引用
孙德日,胡慧媛,朱悦..改良脊髓半横断损伤大鼠模型的建立及评价[J].中国医科大学学报,2017,46(3):223-226,237,5.基金项目
辽宁省自然科学基金(2013021032) （2013021032）
