中国药理学通报2017，Vol.33Issue(3)：366-372,7.DOI:10.3969/j.issn.1001-1978.2017.03.015
雷公藤内酯醇对肝细胞色素P450的诱导及机制研究
Investigation of inductive effect of triptolide on cytochrome P450 s in rat hepatocytes and HepG2 cells and possible mechanism
摘要
Abstract
Aim Toinvestigatingtheinductionof CYPs in hepatocytes or HepG2 cells by triptolide(TP) andthepossiblemechanism.Methods AfterTPtreat-ment,the expression of CYPs in rat primary hepato-cytes or human HepG2 cells was detected by real-time PCR and Western blot assays.Specific inhibitors or gene knockdown method were employed to analyze the possiblemechanism.Results Theexpressionof CYP1A2,2C7,2C11,2C12,2D2,2E1 and 3A1 in rat primary hepatocytes was induced by TP.The fold was 19,2,31,3,21,88 and 34 at 50 nmol·L-1, respectively while at 100 nmol·L-1 it was 20,5,30,23,61,83 and 38,respectively.In HepG2 cells,the expression of human CYP1A1,2B6,2C9,2C19, 2D6,2E1 and 3A4 was also induced by TP.The ac-tivities of nuclear receptor PXR and CAR were inhibi-ted.TP upregulated p53 expression,and the induction of several CYPs caused by TP was blocked when p53 wasinhibited.Conclusions TPinducesCYPsexpres-sion in rat hepatocytes and HepG2 cells.Nuclear re-ceptors may not be involved in TP induced CYPs, while the mechanism may partly attribute to p53.关键词
雷公藤内酯醇/细胞色素P450/核受体/p53/肝细胞/HepG2Key words
triptolide(TP)/cytochrome P450(CYPs)/nuclear receptor/p53/hepatocytes/HepG2分类
医药卫生引用本文复制引用
虞茜,万子衿,朱燕萍,徐海荣,廖凯,刘峰,李巍..雷公藤内酯醇对肝细胞色素P450的诱导及机制研究[J].中国药理学通报,2017,33(3):366-372,7.基金项目
国家自然科学基金资助项目(No 81303109) （No 81303109）
扬州市省高校自然科学基金面上项目(No 14KJB310026) （No 14KJB310026）
扬州市自然科学基金青年科技人才项目(No YZ2014020) （No YZ2014020）
扬州大学大学生创新计划(No x2015764) （No x2015764）
