中国药业2017，Vol.26Issue(3)：8-11,4.DOI:10.3969/j.issn.1006-4931.2017.03.003
蒿甲虚热清颗粒的制备及其含量测定方法研究
Preparation and Content Determination Method of Haojia Xureqing Granules
摘要
Abstract
Objective To simultaneously determinate the content of paeonol and puerarin in Haojia Xureqing Granules with high perfor-mance liquid chromatography method. Improve the efficiency of production and testing. Methods The fluidized bed was used to make granules with the herbal extract as binder. HPLC was performed on the column of YMC-Pack ODS-A C18 ( 250 mm × 4. 6 mm, I. D. S-5μm, 12 nm ) with mobile phase of methanol ( A ) -water ( B ) -0. 2% phosphoric acid solution ( C ) , at flow rate of 1. 0 mL/min, in gradient elution, column temperature was 35 ℃, the injection volume was 10 μL, and determine wavelength was 274 nm. Results Paeonol and pueraria were in good linear relationship ( r=0. 9995 ) in the range of 0. 418 μg-1. 464 μg, 0. 608 μg-2. 126 μg;the RSD of precision, reproducibility tests and the stabilities were no more than 3 %. The scopes of sample recovery rate were 98. 64% -103. 84% ( RSD=1. 93%, n=6 ) , 98. 98% -102. 99% ( RSD=1. 74%, n=6 ) . Conclusion The preparation technology is simple and feasible, the content determination method is simple, accurate and reproducible, and can be used for the quality control of the Haojia Xureqing Granules.关键词
蒿甲虚热清颗粒/一步制粒/高效液相色谱法/丹皮酚/葛根素/含量测定Key words
Haojia Xureqing Granules/one-step granulating method/HPLC/paeonol/puerarin/content determination分类
医药卫生引用本文复制引用
王远苹,余楚钦,杨怡,林华庆..蒿甲虚热清颗粒的制备及其含量测定方法研究[J].中国药业,2017,26(3):8-11,4.基金项目
广东省科技计划项目[2013B021800080]. （）
