中国药业2017，Vol.26Issue(3)：30-32,3.DOI:10.3969/j.issn.1006-4931.2017.03.010
反相高效液相色谱法测定药材腊梅花中芦丁含量
Content Determination of Medicinal Flowers Rutin Chimonanthus by RP-HPLC
黄玮 1肖新月 2吴秀清 2姚强文3
作者信息
- 1. 四川省资阳市食品药品检验检测中心,四川资阳 641399
- 2. 中国食品药品检定研究院,北京 100003
- 3. 四川金辉药业有限公司,四川资阳 641300
- 折叠
摘要
Abstract
Objective To establish an RP-HPLC method for the content determination of medicinal flowers Lamei Rutin. Methods Agilent C18 column(250 mm × 4. 6 mm, 5 μm) was used, the column temperature was 30 ℃, Methanol-0. 05% phosphoric acid aqueous solution ( 35:65 ) , the flow rate was 0. 8 mL/min. Results In the selected chromatographic conditions, the peak area of the common logarithm ( Y ) of the commonly used sample size to number of ( X ) for linear regression, the regression equation was Y=1643977. 49 X+34610. 54, the linear range was 0. 00519-1. 5570 μg ( r2=1. 00 ) , the recovery rate was 98. 91%, RSD was 1. 55%( n=6 ) . Conclusion This method is simple, accurate, reproducible, which can be used for the quality control of Rutin Chimonanthus flowers.关键词
芦丁/腊梅花/反相高效液相色谱法Key words
Rutin/chimonanthus flowers/RP-HPLC分类
医药卫生引用本文复制引用
黄玮,肖新月,吴秀清,姚强文..反相高效液相色谱法测定药材腊梅花中芦丁含量[J].中国药业,2017,26(3):30-32,3.
