中国药业2017，Vol.26Issue(4)：6-8,3.DOI:10.3969/j.issn.1006-4931.2017.04.002
鲁斯可皂苷元制备方法研究
Preparation Method of Ruscogenin
摘要
Abstract
Objective To investigate the method of separating and purifying ruscogenin from Ophiopogon japonicus. Methods 80%ethanol was used to extract the total saponins,two-phase acid hydrolysis of total saponins,silica gel column chromatography separation, methanol recrystallization;TLC,HPLC methods were used to check the purity. Results Ruscogenin was consistent with the control substance by TLC and no impurity spots. The purity was 98. 58% by HPLC. Conclusion The preparation method is simple,safe,feasible with low cost and high efficiency. The obtained ruscogenin can be used as a reference substance for qualitative and quantitative analysis of traditional Chinese medicine.关键词
麦冬/鲁斯可皂苷元/制备/薄层色谱法/高效液相色谱法Key words
Ophiopogon japonicus/ruscogenin/preparation/TLC/HPLC分类
医药卫生引用本文复制引用
曹青云,李琦,王敏,肖顺丽,高荣凯,张宏桂..鲁斯可皂苷元制备方法研究[J].中国药业,2017,26(4):6-8,3.基金项目
国家自然科学基金(31070328). （31070328）
