中国药业2017，Vol.26Issue(4)：27-31,5.DOI:10.3969/j.issn.1006-4931.2017.04.007
离子色谱-积分脉冲安培检测法测定β-环糊精中的有关物质
Determination of Related Substance in Betacyclodextrin by Ion Chromatography with Integral Pulse Amperometric Detection
王淼 1靳贵英 1王彩媚1
作者信息
- 1. 广东省药品检验所,广东广州 510180
- 折叠
摘要
Abstract
Objective To establish a method of ion chromatography with integral pulse amperometric detection for the determination of related substance in betacyclodextrin. Methods Dionex ICS-500 and CarPac PA200 column were used. The mixtures of pure water, 250 mmol/L NaOH solution and 500 mmol/L NaAC solution in different ratio were used as mobile phase in the gradient elution. In the amperometric detection,gold electrode was used as working electrode,Ag/AgCl electrode was used as reference electrode. Results The peaks of different impurities were separated completely. The calibration curve of each solvent of each impurity showed a good liner relationship with good precision and recovery rate. Conclusion The method showed good sensitivity,precision and reproducibility,which can be used to determine the related substance in betacyclodextrin.关键词
β-环糊精/离子色谱/积分脉冲安培检测/有关物质Key words
betacyclodextrin/ion chromatography/integral impulse amperometric detection/related substance分类
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王淼,靳贵英,王彩媚..离子色谱-积分脉冲安培检测法测定β-环糊精中的有关物质[J].中国药业,2017,26(4):27-31,5.
