中国真菌学杂志2017，Vol.12Issue(1)：3-7,5.
烟曲霉孢子对肥大细胞活化作用的研究
Effects of Aspergillus fumigatus spore on the activation of mast cell
摘要
Abstract
Objective To study the etfects of Aspergillus fumigatus spore on the activation of human mast cell line HMC-1.Methods Prepared heat-killed Aspergillusfumigatus spore antigen by heating method.HMC-1 cells were co-cultured with heat-killed Aspergil lus fumigatus spores whose concentration was 5 × 105.Microplate spectrophotometer was applied to the β-hexosaminidase release assay which was an index of mast cell degranulation.Enzyme-linked immunosorbent assay (ELISA) was used for released IL-5.The expression levels of IL-5 mRNA was detected by Realtime-PCR.Results Compared with the negative control group,heat-killed Aspergillus fumigatus spores increased β-hexosaminidase release [(11.3 ％ ± 1.2 ％) VS (0.5％±0.1％),P＜0.05],IL-5 mRNA expression level [(2.47±0.12) VS (0±0.26),P＜0.05] and the release of IL-5 [(11.96±1.09) VS (9±1.36),P＜0.05] in HMC-1 cells.Conclusion Aspergillusfumigatus spores activates mast cell line HMC-1 resulting in cell degranulation,synthesis and release of IL-5.关键词
烟曲霉/孢子/肥大细胞/脱颗粒/白细胞介素-5Key words
Aspergillus fumigatus/spore/mast cell/degranulation/interleukin-5分类
医药卫生引用本文复制引用
陈丽莎,李燕明,佟训靓,赵作涛,王辰..烟曲霉孢子对肥大细胞活化作用的研究[J].中国真菌学杂志,2017,12(1):3-7,5.基金项目
国家十二五科技支撑计划(2012BAI 05B02) （2012BAI 05B02）
