中国真菌学杂志2017，Vol.12Issue(1)：8-12,5.
含PxIxIT模序烟曲霉KpsF基因作用初探
The study of KpsF gene which contains PxIxIT motif in Aspergillus fumigatus
摘要
Abstract
Objective To construct the KpsF gene defect strains △KpsF and to explore the interaction between KpsF gene and calcineurin.Methods Amplificated the upstream and downstream of KpsF about 1.0 kb DNA fragments,the pyrG of plasmid pJW 24 was used as a biomarker when constructting the knockout plasmid.Recombinant plasmid was transformed into A.fumigatus Ku80 pyrG and got △KpsF.To observer the radial and pbenotypic growth of Ku80 and △KpsF,Real-time PCR was used to determine the relative expression of calcineurin catalytic subunit (CnaA) of Ku80 (control group)and △KpsF (experimental group) in GMM liquid medium containing different concentration of CaCl2.Results Compared with Ku80,the radial growth of △KpsF had no obvious change (P＞0.05),and the phenotypic growth had no obvious difference.In GMM Liquid medium containing 10 mmol/L CaCl2,the relative expression of CnaA of △KpsF strain was significantly higher than Ku80 (P＜0.01).However,the relative expression of CnaA did not obviously changed (P＞0.05) in GMM Liquid medium containing 100 mmol/L CaCl2.Conclusions The KpsF had no effect in radial and phenotypic growth of A.fumigatus.Under the condition of different concentrations of Ca2+,the relative expression levels of CnaA gene were different between △KpsF and Ku80,suggesting that the KpsF might be involved in the feedback regulation of calcineurin under the condition of low concentration of CaCl2.关键词
烟曲霉/钙调磷酸酶/KpsF基因/PxIxIT模序Key words
Aspergillus fumigatus/Calcineurin/KpsF gene/PxIxIT motif分类
医药卫生引用本文复制引用
李佳娟,李雯,马彦..含PxIxIT模序烟曲霉KpsF基因作用初探[J].中国真菌学杂志,2017,12(1):8-12,5.基金项目
国家青年科学基金(81101233),山西省青年科技研究基金(2010021036-2),山西省卫生计生委科研课题(2015043),山西省回国留学人员科研资助项目(2016-052) （81101233）
