中国真菌学杂志2017，Vol.12Issue(1)：13-18,6.
白头翁汤正丁醇提取物诱导白念珠菌生物被膜细胞凋亡
Butyl alcohol extract of BaiTouWeng decoction induced apoptosis in Candida albicans biofilms
施高翔 1汪云霞 2冯鑫 1邵菁 2汪天明 1汪长中2
作者信息
- 1. 安徽中医药大学中西医结合临床学院,合肥230038
- 2. 安徽省中医药科学院中西医结合研究所,合肥230038
- 折叠
摘要
Abstract
Objective To explore the Butyl alcohol extract of Bai Tou Weng decoction effect on apoptosis of Candida albicans biofilms.Methods Using DCFH-DA staining flow cytometry to detect Candida albicans biofilm cells of reactive oxygen species (ROS) level;Using JC-1 staining of mitochondria of Candida albicans biofilm membrane flow cytometry to detect MMP;Phosphatidylserine externalization was recorded by fluorescence microscopic with Annexin V staining at the early stage of apoptosis in C.albicans cells of biofilms;Metacaspase activity was observed by Fluorescence microscopic with FITC-VAD-FMK staining at the early stage of apoptosis in C.albicans cells of biofilms;Observation on Candida albicans biofilm morphology was performed by DAPI nuclear staining and fluorescence microscopy.Results The apoptosis of Candida albicans significantly increased the proportion of more than 128 ug mL-1BAEB treatment,Caspase activity increased,nuclear pyknosis and fragmentation were observed.Intracellular ROS level was significantly increased,mitochondrial membrane potential decreased significantly.Conclusion The Butyl alcohol extract of BaiTou Weng decoction of certain concentrations could induce apoptosis of Candida albicans biofilm cells.关键词
白头翁汤正丁醇提取物/白念珠菌/生物被膜/凋亡Key words
Butyl alcohol extract of Bai Tou Weng decoction/C.albicans/biofilms/apoptosis分类
医药卫生引用本文复制引用
施高翔,汪云霞,冯鑫,邵菁,汪天明,汪长中..白头翁汤正丁醇提取物诱导白念珠菌生物被膜细胞凋亡[J].中国真菌学杂志,2017,12(1):13-18,6.
