中国真菌学杂志2017，Vol.12Issue(1)：19-22,18,5.
MMP9、S100A10基因沉默稳转小鼠细胞系的构建及验证
Construction and verification of stable down-regulated MMP9, S100A10 mouse brain microvascular endothelial cell lines
摘要
Abstract
Objective To construct stable down-regulated MMP9,S100A10 mouse brain microvascular endothelial cell lines,then verify the reduction level of gene expression.Methods By transfecting LV-musMMP9-shRNA,LV-musS100A10-shRNA into b.End3 respectively,then the very cell lines were screened by puromycin.The obtained differential b.End3 cell lines were observed by fluorescence microscope and varified by RT-qPCR.Results The study successfully constructed stable transfected cell lines which could effectively interference S100A10,MMP-9 expression of 3 × 108 TU/mL and 1 × 108 TU/mL LV-musS100A10-shRNA respectively.Conclusions The targeted down-regulation b.End3 cell lines are useful for further exploring the role of molecule S100A10 or MMP-9 in host blood-brain barrier by control variables and the possible target of treatment exploration of cryptococcal encephalitis/meningitis.关键词
慢病毒载体/基质金属蛋白酶9 (MMP9)/S100A10/b.End3Key words
Lentivirus vector/MMP9/S100A10/b.End3分类
医药卫生引用本文复制引用
陈雪雯,朱红梅,温海..MMP9、S100A10基因沉默稳转小鼠细胞系的构建及验证[J].中国真菌学杂志,2017,12(1):19-22,18,5.基金项目
国家自然科学基金(31470252,81271800,31270181) （31470252,81271800,31270181）
