浙江医学2017，Vol.39Issue(1)：16-19,4.
颅脑创伤后大鼠脑组织自噬表达变化及渥曼青霉素保护作用的研究
Expression of autophagy-related proteins in brain tissue and neuroprotective effect of wortmannin after traumatic cerebral injury in rats
摘要
Abstract
Objective To investigate the expression of autophagy-related proteins in brain tissues and the neuroprotec-tive effect of wortmannin after traumatic cerebral injury in rats.Methods One hundred and twenty healthy adult male SD rats were randomly divided into 3 groups:the sham operation group(A),the traumatic brain injury group(B) and the wortmannin group (C) with 40 animals in each group.Precision cortical impactor was used to establish medium traumatic brain injury model in group B and C with a hit point of 4mm in diameter.The rats were pretreated with a single intracerebroventricular injection of wortmannin in group C,while with injection of DMSO in group B.The rats in group A received surgery to expose the dura without actual impact,and were injected PBS before the modeling.The expression of microtubule-associated protein 1 light chain3 B (LC3-Ⅱ) and p62 in brain tissues were detected by Western blot.The neurologic function of rats was assessed with behavioral score.Water content was evaluated by dry-wet analysis.Results The expression of LC3-Ⅱ was increased in groups B and C compared with group A at 24h,72h and 168h after injury,the expression of p62 was decreased,and the ratio of LC3-Ⅱ/LC3-Ⅰ was increased (all P＜0.05).The expression of LC3-Ⅱ was lower in group C than that in group B at 24h,72h and 168h after injury,the expression of p62 was higher (all P＜0.05) in group B and the ratio of LC3-Ⅱ/LC3-Ⅰ was decreased at 72h and 168h (all P＜0.05) compared to group C.At 48h and 72h after injury,the behavioral scores in group C were significantly higher than that in group B (all P＜0.05).At 72h,the water contents of brain tissue in group B and C were higher than that in group A,while the water content in group C was significantly lower than that in group B(P＜0.05).Conclusion Wortmannin can suppress the excessive autophagy,reduce the brain edema induced by traumatic cerebral injury and improve the neurological function in rats.关键词
颅脑创伤/自噬相关蛋白/渥曼青霉素Key words
Traumatic brain injury/Autophagy related proteins/Wortmannin引用本文复制引用
汤崇辉,方战舰,胡益岚,童云,邬芬赞,傅小君,魏晓捷..颅脑创伤后大鼠脑组织自噬表达变化及渥曼青霉素保护作用的研究[J].浙江医学,2017,39(1):16-19,4.基金项目
宁波市自然科学基金项目(2015A610192) （2015A610192）
慈溪市科技计划项目(CN2015022) （CN2015022）
