浙江医学2017，Vol.39Issue(1)：20-23,27,5.
EGFR抑制剂对人结肠癌细胞株HT29中CD44+细胞及侧群细胞作用的研究
Effect of epidermal growth factor receptor inhibitor on CD44+ cells and side population cells of human colon cancer cell line HT29
摘要
Abstract
Objective To explore the effect of epidermal growth factor receptor (EGFR) inhibitor on CD44+ and side population (SP) cells in colon cancer HT-29 cells.Methods The proportion and tumorigenicity of CD44+ cells and SP cells was detected by Hoechst33342 staining and flow cytometry.The effects of EGFR inhibitor gefitinib at different concentrations on the sphere formation and the proportion of CD44+ cells and SP cells were analyzed.Results EGFR inhibitor significantly inhibited the growth of CD44+ cells and SP cells.The proportion of CD44+ cells in HT29 cells was decreased after treatment with gefitinib for 24h and 48h.The proportion of SP cells in HT29 cells was decreased from (3.82 ± 0.08)％ to (0.34 ± 0.03)％ after verapamil treatment.It was also decreased significantly after gefitinib treatment:Gefitinib had an inhibitory effect on the sphere formation in HT29 cells.Conclusion There are CD44+ and SP cells subpopulation in colon cancer cells.EGFR inhibitor can inhibit the growth of CD44+ and SP cells in colon cancer HT-29 cells,which may provide information for targeting therapy of colon cancer.关键词
CD44+/侧群细胞吉非替尼/结肠癌Key words
CD44+/Side-population cells/Gefitinib/Colon cancer引用本文复制引用
巩超捷,唐燕,夏璐..EGFR抑制剂对人结肠癌细胞株HT29中CD44+细胞及侧群细胞作用的研究[J].浙江医学,2017,39(1):20-23,27,5.基金项目
上海市自然科学基金(09ZR1418700) （09ZR1418700）
