浙江医学2017，Vol.39Issue(1)：24-27,4.
Thr187磷酸化p27kip1与人乳腺癌MCF-7细胞化疗敏感性的关系
Relationship of p27kip1 Thr187 phosphorylation with chemosensitivity of breast cancer MCF-7 cells
摘要
Abstract
Objective To investigate the relationship of p27kip1 Thr187 phosphorylation(P-p27Thr187) with chemosensitivity of human breast cancer MCF-7 cells.Methods Cultured MCF-7 cells were treated with epirubicin (EPI),docetaxel (DOC),5-fluorouracil (5-Fu),cyclophosphamide (CTX) and cisplatin (CDDP),the inhibitory rates were measured by MTT method.The expression of P-p27Thr187 and p27kip1 was detected by Western blot.The conjugation of P-p27Thr187 and S-phase kinase-associated protein 2 (Skp2) was detected by co-immunoprecipitation method.Results The MTT assay showed that inhibitory effect of 5 chemotherapeutical agents on MCF-7 cells in a dose-dependent manner,and with an order of inhibitory effect as:EPI,DOC,5-Fu,CTX and CDDP.Western blot assay showed that after drugs treatment,the expression of P-p27Thr187 protein was significantly increased,while the expression of p27kip1 was decreased.Co-immunoprecipitation revealed that the conjugation of P-p27Thr187 and Skp2 protein was increased significantly after the chemotherapy drugs treatment.Conclusion P-p27Thr187 protein might be related to the chemosensitivity in human breast cancer MCF-7 cells,and it could be used as a marker for clinical assessment of sensitivity to chemotherapy.关键词
乳腺癌/p27kip1/磷酸化/化疗敏感性Key words
Breast cancer/p27kip1/Phosphorylation/Chemosensitivity引用本文复制引用
郭志琴,吕青山,袁琳娜,王振,陆宁,柯萍,邬万新..Thr187磷酸化p27kip1与人乳腺癌MCF-7细胞化疗敏感性的关系[J].浙江医学,2017,39(1):24-27,4.基金项目
浙江省医学重点学科建设计划项目(GJJX-010-001) （GJJX-010-001）
嘉兴市科技计划项目(2013AY21042-3) （2013AY21042-3）
嘉兴市医学重点支撑学科建设计划项目(04-Z-01) （04-Z-01）
嘉兴市第一医院“启明星”计划项目(2013QMX003) （2013QMX003）
