浙江医学2017，Vol.39Issue(1)：32-36,5.
NLRP3炎症小体通路在慢性阻塞性肺病发展中的作用研究
Association of NLRP3 inflammasome signaling pathway with progression of chronic obstructive pulmonary disease
摘要
Abstract
Objective To study the association of NLR pyrin domain-containing protein 3 (NLRP3) inflammasome signaling pathway with the progression of chronic obstructive pulmonary disease (COPD).Methods Ninety four COPD patients,including 31 cases of stage Ⅱ (moderate),33 cases of stage Ⅲ (severe) and 30 cases of stage Ⅳ (extremely severe),were enrolled in the study.The pulmonary function was examined in patients both with stable and acute exacerbation stages.The expression of NLRP3,ASC,Casp-1,IL-1 β,IL-18 mRNA in peripheral blood mononuclear cells (PBMCs) were determined by real-time PCR.The concentrations of cytokines IL-1 β and IL-18 in serum were measured by ELISA.The correlation between the levels of NLRP3,ASC,Casp-1,IL-1β,IL-18 mRNA and pulmonary function was evaluated.Results Significantly higher levels of NLRP3,ASC,Casp-1,IL-1 β,IL-18 mRNA transcripts in PBMC from severe and extremely severe COPD groups were found both in the stable and acute exacerbation stage COPD patients,compared with moderate COPD group (all P＜0.05).And the above indexes in extremely severe COPD group were higher than those in severe COPD patients (all P＜0.05).Significantly higher levels of NLRP3,ASC,Casp-1,IL-1β,IL-18 mRNA transcripts in PBMC from acute exacerbation stage COPD patients were also found in moderate,severe and extremely Severe COPD groups compared with stable stages COPD group patients (all P＜ 0.05).Higher serum IL-1β and IL-18 levels in severe and extremely severe COPD groups were found both in the stable and acute exacerbation stage COPD patients compared with moderate COPD group (all P＜0.05).And the IL-1 β and IL-18 levels in extremely severe COPD group were higher than those in severe COPD group (both P＜0.05).Higher serum levels of IL-1 β and IL-18 levels from acute exacerbation stage COPD patients were also found in moderate,severe and extremely Severe COPD groups compared with stable stages COPD group patients (all P＜0.05).The increased levels of NLRP3,ASC,Casp-1,IL-1β,IL-18 mRNA transcripts in PBMC were negatively correlated with the values of FEV1,and FEV1/FVC in stable stages COPD group patients (all P＜0.01).Conclusion NLRP3 inflammasome activation is associated with the acute exacerbation and deterioration of pulmonary function and involved in the progression of COPD.关键词
慢性阻塞性肺病/炎症小体/核苷酸结合寡聚化结构域样受体蛋白3/接头蛋白凋亡相关点样蛋白/半胱天冬酶1/肺功能Key words
Chronic obstructive pulmonary disease/Inflammasome/NLRP3/ASC/Caspase-1/Pulmonary function引用本文复制引用
王师,王华英,翁跃颂,兰朋训,姜昊,俞万钧,应可净..NLRP3炎症小体通路在慢性阻塞性肺病发展中的作用研究[J].浙江医学,2017,39(1):32-36,5.基金项目
宁波市自然科学基金项目(2013A610237) （2013A610237）
