浙江医学2017，Vol.39Issue(2)：89-92,101,5.
儿童急性B淋巴细胞白血病致癌基因FGFR3表达对预后的影响研究
Expression of FGFR3 gene in childhood acute B lymphoblastic leukemia and its relation to disease progression
摘要
Abstract
Objective To investigate the expression of fibroblast growth factor receptors 3 (FGFR3) gene in childhood acute B lymphoblastic leukemia (B-ALL),and its clinical significance in disease progression.Methods The expression levels of FGFR3 in 52 children with B-ALL and 12 normal children were assayed by real time RT-PCR.Clinical indicators were compared between patients with high FGFR3 expression and those with low FGFR3 expression.Patients were followed up for 36 months.The event-free survival (EFS) rate was compared with single factor analysis between two groups.B lymphocyte immune classification was detected by flow cytometry and B lymphocyte fusion gene was detected by nested PCR.The expression levels of FGFR3 were compared among different immune classification and fusion gene groups.Results FGFR3 was over-expressed in children with B-ALL compared with normal controls (1.637 ± 0.903 vs 0.645 ±0.559,P＜0.05).There were no significant differences in sex,age,peripheral blood WBC count,hepatosplenomegaly,extramedullary infiltration,B lymphocyte immune classification and karyotype between low FGFR3 group and high FGFR3 group (P ＞0.05).The probability of 3-year EFS for patients with high FGFR3 level was significantly lower than that with low FGFR3 level (49.97％ vs 76.17％,P＜0.05).Expression levels of FGFR3 were significantly different between BCR/ABL+ group and BCR/ABL-group (P＜0.05) and between CD34+ group and CD34-group (P＜0.05).Conclusion Over-expression of FGFR3 may participate in malignant hematopoiesis and the detection of FGGR3 expression might be helpful in evaluation of disease progression in childhood acute B lymphoblastic leukemia.关键词
急性B淋巴细胞白血病/FGFR3/预后Key words
Acute B lymphoblastic leukemia/Fibroblast growth factor receptor 3/Progression引用本文复制引用
吴静怡,周剑峰,裴仁治,张丕胜,刘旭辉,杜小红..儿童急性B淋巴细胞白血病致癌基因FGFR3表达对预后的影响研究[J].浙江医学,2017,39(2):89-92,101,5.基金项目
浙江省自然科学基金资助项目(LQ12H08001) （LQ12H08001）
浙江省医药卫生科技项目(2012KYB186) （2012KYB186）
宁波市自然科学基金项目(2012A610236) （2012A610236）
宁波市医学科技计划项目(2011B23) （2011B23）
