浙江医学2017，Vol.39Issue(3)：152-155,4.
神经病理性疼痛大鼠脊髓背角Sirt1与miR-34b表达的变化
Expression of Sirt1 and miR-34b in spinal dorsal horn of rats with neuropathic pain
摘要
Abstract
Objective To investigate the expression of Sirt1 and miR-34b in spinal cord of rats with neuropathic pain.Methods Fourteen male SD rats were randomly divided into sham operation group and sciatic nerve chronic constriction injury (CCI) group with 7 rats in each group.The mechanical withdrawal threshold (MWT) and thermal paw withdrawal threshold (TWL) of rats were determined at 1 d before and 7,14d after establishment of animal model.At d14 of CCI operation the animals were sacrificed and L4～5 segment of spinal cord was obtained,and the morphological changes of motor neurons in the dorsal horn of the spinal cord were observed with HE staining.Real-time PCR was used to detect the expression of Sirt1 and miR-34b in the dorsal horn of the spinal cord,and the protein expression of Sirt1,BDNF and CREB in rat spinal dorsal horn was determined by Western blot.Results The rat model of CCI were successfully established as tested by MWT and TWL.Histopathological observation showed that the number of spinal dorsal horn neurons was significantly decreased in CCI group after 7d and 14d (P＜0.05).Real-time PCR showed that Sirt1 expression was decreased and miR-34b expression was increased in CCI group compared with the control group (P＜0.05).Western blot showed that the expression of Sirt1 and CREB decreased,and the expression of pCREB and BDNF increased.Conclusion The expression changes of Sirt1 and miR-34b may be involved in the formation and maintenance of neuropathic pain induced by chronic constriction injury of sciatic nerve.关键词
神经病理性疼痛/CCl/Sirt1/miR-34b/脊髓背角Key words
Neuropathic pain/Chronic constriction injury/Sirt1/miR-34b/Dorsal horn of spinal cord引用本文复制引用
郑晖,杨柳英,张娟,郭小文,陶涛,尉明洋,施冬生,马千..神经病理性疼痛大鼠脊髓背角Sirt1与miR-34b表达的变化[J].浙江医学,2017,39(3):152-155,4.基金项目
浙江省中医药科学研究基金项目(2015ZB052) （2015ZB052）
