浙江医学2017，Vol.39Issue(4)：245-249,5.DOI:10.12056/j.issn.1006-2785.2017.39.4.2016-1489
雌激素对去卵巢大鼠骨组织Wnt16、 β-catenin 、 OPG 、RANKL表达的影响
Effects of estrogen on Wnt16, β-catenin, OPG, RANKL expression in bone tissue of ovariectomized rats
摘要
Abstract
Objective To investigate the effects of estrogen on the expression of Wnt16,β-catenin,OPG,RANKL in bone tissue of ovariectomized rats.Methods Forty female Wistar rats were randomly divided into sham operation group,ovariectomized group,estrogen group 1 and estrogen group 2.In the estrogen groups,17 β-E2 was injected subcutaneously for 4 weeks or 16 weeks after ovariectomy,respectively.The other two groups were given the same volume of normal saline.The expression of Wnt16 mRNA was detected with RT-PCR,the expressions of OPG,RANKL,β-catenin proteins in bone cortex tissue were detected with Western blot and immunohistochemistry,respectively.The thickness of the trabecular bone (Tb.Th),number of trabecular bone (Tb.N) and thetrabecular spacing (Tb.SP) were measured using HE staining analysis,and serum E2 level was determined by ELISA.Results Compared to ovariectomized group,The expression of OPG,β-catenin,Wnt16 mRNA in bone tissue,the serum E2 levels,and Tb.Th,Tb.N in estrogen group 2 and sham operation group were significantly increased,the expression of RANKL and Tb.SP were significantly decreased(all P＜0.05).Compared to ovariectomized group the expression of β-catenin,serum E2,and Tb.Th,Tb.N in estrogen group 1 were significantly increased,the expression of RANKL and Tb.SP were significantly decreased (all P＜0.05).Conclusion Estrogen may prevent osteoporosis by up-regulation of Wnt16,β-catenin,OPG and down-regulation RANKL expression in bone tissue.关键词
雌激素/Wnt16/β-catenin/OPG/RANKL/骨质疏松Key words
Estrogen/Wnt16/β-catenin/OPG/RANKL/Osteoporosis引用本文复制引用
魏双双,张治芬,黄哲人,何轶然,吴红艳,刘文华,汤珊珊,黄坚..雌激素对去卵巢大鼠骨组织Wnt16、 β-catenin 、 OPG 、RANKL表达的影响[J].浙江医学,2017,39(4):245-249,5.基金项目
国家卫生计生委科学研究基金-浙江省医药卫生重大科技计划(WKJ-ZJ-024) （WKJ-ZJ-024）
浙江省科技厅重大科技专项重点项目(2014C03044-1) （2014C03044-1）
杭州市医药卫生科技计划重点项目2011Z003) （）
杭州市科技局重点项目(20142013A58,20140733Q16) （20142013A58,20140733Q16）
