浙江医学2017，Vol.39Issue(4)：255-258,4.DOI:10.12056/j.issn.1006-2785.2017.39.4.2014-1439
左乙拉西坦对癫痫小鼠内嗅皮层GABA神经元电生理干预的研究
Electrophysiological changes of GABAergic neurons of entorhinal cortex in epileptic mouse after levetiracetam intervention
摘要
Abstract
Objective To investigate the electrophysiological changes of GABAergic neurons in entorhinal cortex of epileptic mouse after levetiracetam intervention.Methods Transgenic FVB-Tg mice were randomly divided into control group,model group,and levetiracetam group.The epilepsy model was induced by sequential injection of scopolamine and pilocarpine in TVB-Tg mice.Levetiracetam was given by intragastric administration.Tissue specimens of entorhinal cortex were obtained after animals were sacrificed.The brain slices (400μm) were prepared and incubated in oxygenated artificial cerebrospinal fluid.The sequential spike patters(inter-spike intervals,ISI) and intrinsic properties (ARP,Vts,Vr) of GABAergic neurons were recorded by the whole-cell clamp and analyzed with pClamp 9.Results Compared to control groups,in model group the ARP was prolonged,the Vts-Vr increased,and the ISI was extended (all P＜0.05).Compared to model group,in levetiracetam group the ARP were significantly shortened (P＜0.05),the threshold potentials significantly decreased (P＜0.01),and the ISI was shortened (P＜0.05).Conclusion With the intervention of levetiracetam,the spike encoding and intrinsic properties of GABAergic neurons were improved in epileptic mice,which suggests that the functional enhancement of GABAergic neurons may play an important role in epileptic control.关键词
左乙拉西坦/癫痫/内嗅皮层/GABA神经元Key words
Levetiracetam/Epileptic/Entorhinal cortex/GABAergic neuron引用本文复制引用
倪红,郑卜真,王恩,马传花,柯绍发,周元林,金笑平,储照虎..左乙拉西坦对癫痫小鼠内嗅皮层GABA神经元电生理干预的研究[J].浙江医学,2017,39(4):255-258,4.基金项目
台州市科技局资助项目(121ky08) （121ky08）
