浙江医学2017，Vol.39Issue(4)：259-262,276,5.DOI:10.12056/j.issn.1006-2785.2017.39.4.2016-1304
沉默Calnexin基因表达对胃癌细胞SGC-7901内质网应激凋亡及其信号通路的影响
Effects of calnexin silencing on endoplasmic reticulum stress-apoptosis and its signaling pathway in gastric cancer cell line SGC-7901
摘要
Abstract
Objective To investigate the effects of calnexin gene silencing on endoplasmic reticulum stress-apoptosis and its signaling pathway in gastric cancer SGC-7901cells.Methods L.entiviral vectors for short hairpin RNAs targeting the coding region of human calnexin gene were constructed.The recombinant lentiviral vectors were harvested from 293T cells and were used to transfect human gastric cancer SGC-7901 cells.SGC-7901 cells were transfected with shRNA-Calnexin (experimental group) or shRNA blank plasmid (blank vector group),and the non-transfected SGC-7901 cells were used as control group.Expression of calnexin mRNA in SGC-7901 cells were detected by RT-PCR,cell proliferation was detected by MTT,cell apoptosis was detected by flow cytometry,expression of GRP94,IRE1,ATF6 and CHOP were detected by Western blot.Results The expression of Calnexin mRNA was down-regulated in experimental group compared to control group (P＜ 0.05),and the cell proliferation was significantly decreased (P＜0.05).The apoptosis rate was increased 72h after transfection,compared with the control group (P＜0.05).The expression of GRP94,IRE1,ATF6 and CHOP was significantly increased in experimental group(P＜0.05).Conclusion Calnexin silencing can inhibit cell proliferation and induce apoptosis of gastric cancer SGC-7901 cells probably by inhibiting endoplasmic reticulum stress signaling pathway.关键词
钙连蛋白/SGC-7901细胞/慢病毒/内质网应激/凋亡/胃癌Key words
Calnexin/SGC-7901 cell/Slow virus/Endoplasmic reticulum stress/Apoptosis/Gastric cancer引用本文复制引用
张晓海,张洪涛,胡孝定,钟华..沉默Calnexin基因表达对胃癌细胞SGC-7901内质网应激凋亡及其信号通路的影响[J].浙江医学,2017,39(4):259-262,276,5.基金项目
浙江省医药卫生科研项目(2013KYB076) （2013KYB076）
