浙江医学2017，Vol.39Issue(4)：263-265,3.DOI:10.12056/j.issn.1006-2785.2017.39.4.2016-966
结缔组织生长因子在肾积水模型大鼠肾组织中的表达研究
Expression of CTGF in kidney tissue of rat hydronephrosis model
刘永东 1韩建强 1张广平 2李俊峰 1张传光 1胡强1
作者信息
- 1. 261041 山东省潍坊市人民医院小儿外科
- 2. 山东省兰陵县人民医院小儿外科
- 折叠
摘要
Abstract
Objective To investigate the expression of connective tissue growth factor (CTGF) in kidney tissue of rat hydronephrosis model.Methods Forty eight healthy male Wistar rats were equally divided into two groups:in model group the left pelviureteric junction was dissociated and the ureter was ligated,and in control group the left pelviureteric junction was dissociated only.Six rats in each group were sacrificed 1,2,4 and 8 weeks after the operation and the left kidney was taken for histopathological examination.The expression of CTGF protein and mRNA in kidney tissue was detected with immunohistochemistry and Western blot,respectively.Results The expression of CTGF mRNA and protein in model group was significantly higher than that in the control group (P＜0.01) and increased with the time length of pelviureteric junction obstruction.Conclusion The expression of CTGF is upregulated in the rat hydronephrosis model and positively correlated with the obstruction duration,indicating that CTGF might be used as a target of preventing congenital hydronephrosis.关键词
大鼠/肾积水/结蹄组织生长因子Key words
Rats/Hydronephrosis/CTGF引用本文复制引用
刘永东,韩建强,张广平,李俊峰,张传光,胡强..结缔组织生长因子在肾积水模型大鼠肾组织中的表达研究[J].浙江医学,2017,39(4):263-265,3.
