肿瘤防治研究2017，Vol.44Issue(2)：98-102,5.DOI:10.3971/j.issn.1000-8578.2017.02.004
miR-494下调Sox9的表达对骨肉瘤细胞MG-63增殖和侵袭的影响
miR-494 Inhibits Proliferation and Invasion of Osteosarcoma Cells MG-63 Through Downregulating Sox9 Expression
摘要
Abstract
Objective To investigate the impact of miR-494 on the proliferation and invasion of human osteosarcoma cells MG-63 and to confirm whether Sox9 is the target gene of miR-494.Methods MG-63 cells were transfected with recombinant adenovirus miR-494(Ad-miR-494),CCK-8 and clone formation experiments were employed to determine the proliferation ability of transfected MG-63 cells.Cells invasion abilities were determined by Transwell assay.Real-time PCR was used to analyze the expression of Sox9 mRNA and protein level to confirmthe adenovirus miR-494 expression in MG-63 cells.The protein expression of Sox9 was analyzed by Western blot.Results The expression ofmiR-494 was increased obviously (t=36.78,P=0.000) in MG-63 cells which were transfected with Ad-miR-494.CCK-8,clone formation and Transwell experiment results revealed that the proliferation (F=l.711,P=0.012),clone formation (F=2.742,P=0.019)and invasion (F=1.653,P=0.006) abilities of MG-63 cells were markedly inhibited by the overexpression of miR-494.In addition,we observed the decreased Sox9 protein (F=5.827,P=0.021) and mRNA expression (F=5.827,P=0.021) in MG-63 cells which were transfected with Ad-miR-494.And the proliferation (t=27.54,P=0.042),lone formation (t=29.64,P=0.026) and invasion (t=32.48,P=0.016) abilities of MG-63 cells were markedly inhibited by the decreases of Sox9 expression.Conclusion MG-63 cells proliferation and invasion abilities are suppressed by miR-494,and this process is potentially achieved via suppressing Sox9 expression.关键词
miR-494/骨肉瘤/增殖/侵袭Key words
miR-494/Osteosarcoma/Proliferation/Invasion分类
医药卫生引用本文复制引用
李宝林,邓正华,常欧,曾章锐,刘靳波..miR-494下调Sox9的表达对骨肉瘤细胞MG-63增殖和侵袭的影响[J].肿瘤防治研究,2017,44(2):98-102,5.基金项目
2014年四川省科学技术厅科研专项基金(14JC0801) （14JC0801）
2016年泸州市科技局项目科研专项基金(15193) （15193）
