低氧对前列腺癌PC3细胞上皮间质转化的影响OACSCDCSTPCD
Effect of Hypoxia on Epithelia-mesenchymal Transition of Prostate Cancer PC3 Cells
目的 探讨低氧对前列腺癌细胞上皮间质转化的影响.方法 分别在常氧(常氧组)和低氧(低氧组)条件下培养PC3细胞24 h,细胞增殖MTT实验检测低氧对前列腺癌PC3细胞增殖力的影响,Transwell侵袭实验评估低氧对前列腺癌PC3细胞侵袭力的影响,Western biot检测HIF-1α、E-cadherin、N-cadherin和Vimentin的表达水平.另外,在常氧条件下用siRNA抑制HIF-1α表达(干扰组),用Western blot…查看全部>>
Objective To investigate the effect of hypoxia on epithelia-mesenchymal transition of prostate cancer cells PC3.Methods PC3 cells were cultured under aerobic condition (Normal group) and hypoxia condition (Hypoxia group) for 24 h,respectively.Then,the ability of proliferation was detected by MTT,the ability of invasion was assayed by Transwell,and the protein expressions of HIF-1α,E-cadherin,N-cadherin and Vimentin were assayed by Western blot.Meanwhile,PC3 …查看全部>>
刘桂勇;宋洪飞;刘修恒;王敏;魏少忠
433100 潜江,湖北省潜江市中心医院泌尿外科433100 潜江,湖北省潜江市中心医院泌尿外科430060 武汉,武汉大学人民医院泌尿外科430060 武汉,武汉大学人民医院泌尿外科430079 武汉,湖北省肿瘤医院泌尿外科
医药卫生
低氧低氧诱导因子-1α前列腺癌上皮间质转化
HypoxiaHIF-1αProstate cancerEpithelia-mesenchymal transition
《肿瘤防治研究》 2017 (2)
108-111,4
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