作物学报2017，Vol.43Issue(3)：315-323,9.DOI:10.3724/SP.J.1006.2017.00315
OsWR2-RNAi对水稻角质层生物合成和耐旱性的影响
Influence ofOsWR2-RNAi on Rice Cuticle Biosynthesis and Drought Resis-tance
摘要
Abstract
Plant cuticle plays an important role on decreasing plant non-stomatal water loss under drought stress. Overexpression of wax related transcription factor geneOsWR2 in rice can increase total cuticular wax and cutin amounts, affect cuticular wax and cutin monomer composition, and alter cuticular wax crystallization patterns and cuticle membrane ultrastructure, implying important roles ofOsWR2 in rice cuticle biosynthesis and non-stomatal water loss regulation. In this report, we constructed OsWR2RNA inhibition vector (OsWR2-RNAi), and transformed it into rice callus to obtainOsWR2 knockout rice mutants using Agrobacterium-mediated transforming method.OsWR2-RNAi transgenic rice exhibited reduced total cuticular wax amounts by 14.8% mainly due to the decrease of aldehydes, alkanes and alcohols, and lessened full cutin monomer level by 36.2% due pri-marily to the decrease of C16:0ω-OH, C18:1ω-OH, and di-OH fatty acid components.OsWR2-RNAi transgenic rice also showed enriched leaf chlorophyll leaching, water loss rates and MDA contents, whereas reduced tolerance to water deficit. All these physiologic and biochemical index to support the previous hypothesis of OsWR2 acting as a transcriptional regulator of both cuti-cle biosynthetic pathways and non-stomatal water loss, providing evidence ofOsWR2 exerting direct influence over rice dehydra-tion, and a potential application resource in genetic improvement of crop drought tolerance.关键词
OsWR2/RNAi/植物角质层/干旱胁迫/非气孔性失水Key words
OsWR2/RNAi/Plant cuticle/Drought stress/Non-stomatal water loss引用本文复制引用
王莎,贺勇,罗光宇,姚敏,张旭,陈信波,周小云..OsWR2-RNAi对水稻角质层生物合成和耐旱性的影响[J].作物学报,2017,43(3):315-323,9.基金项目
本研究由湖南省重点研发计划项目(2015JC3102),湖南农业大学大学生创新计划项目(XCX1504)和国家自然科学基金项目(31300216)资助. This study was supported by the Key Research Projects of Hunan Province (2015JC3102), the College Students Innovation Project of Hunan Agricultural University (XCX1504), and the National Natural Science Foundation of China (31300216). （2015JC3102）
