作物学报2017，Vol.43Issue(3)：324-331,8.DOI:10.3724/SP.J.1006.2017.00324
转CP4-EPSPs基因大豆杂交后代对草甘膦的抗性水平与遗传背景的相关性
Correlation between Resistance to Glyphosate and Genetic Background in TransgenicCP4-EPSPsGene Soybean Progeny
摘要
Abstract
The first Roundup Ready (RR) soybean (GTS40-3-2) carryingCP4-EPSPsis one of the transgenic crops that have the longest application time and largest planting acreage worldwide. However, it has not been commercialized and has less report in China. In this study, six RR soybean lines derived from GTS40-3-2 were hybridized to create four combinations. We used resis-tance grading and relative plant height in identifying the resistance to glyphosate of parents and F2:3 progeny, to investigate the correlation between resistance of the progeny and genetic background. The growth inhibition of transgenic parents and progeny at seeding stage was not significant when the glyphosate treatment concentration was 1230 g a.i. hm–2, while it was significant when the glyphosate concentration was 3690 g a.i. hm–2 and 4920 g a.i. hm–2. ZLHJ06-1568, ZLHJ10-713, and ZLHJ06-698 among the six parents had the more glyphosate resistance relatively. The progeny of ZLHJ10-713 × ZLHJ06-698 combination had the least inhibition of plant height showing highest resistance to glyphosate in all four F2:3 combinations. The progeny had better resistance to glyphosate than their parents showing better heterosis. The glyphosate resistance of progeny was positively correlated with that of parents. Difference of glyphosate resistance was found in progeny among different combinations because of the interaction between parents. Our study indicates that the expression of glyphosate resistance gene is closely related to the genetic background in soybean, providing a theoretical evidence for target gene selection in developing new varieties by using transgenic germplasm.关键词
转基因大豆/抗草甘膦特性/遗传规律Key words
Transgenic soybean/Glyphosate-resistant characteristics/Inheritance引用本文复制引用
孙如建,孙宾成,张琪,胡兴国,郭荣起,郭兵福,马岩松,于平,张晓莉..转CP4-EPSPs基因大豆杂交后代对草甘膦的抗性水平与遗传背景的相关性[J].作物学报,2017,43(3):324-331,8.基金项目
本研究由国家转基因生物新品种培育重大专项(ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001)资助.This study was supported by the Major Project of China on New Varieties of GMO Cultivation (ZX2008004-001, ZX2011004-001, ZX2013004-001, ZX2014004-001). （ZX2008004-001,ZX2011004-001,ZX2013004-001,ZX2014004-001）
