作物学报2017，Vol.43Issue(3)：343-353,11.DOI:10.3724/SP.J.1006.2017.00343
云南哈尼梯田当前栽培水稻ALK基因的遗传多样性及与稻米糊化温度的关联分析
Genetic Diversity ofALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani's Terraced Fields in Yunnan Province
摘要
Abstract
Yunnan province is one of the centers for genetic diversity of cultivated rice in Asia. A total of 111 rice landraces were collected from Hani's rice terraces of Yunnan, and their population structure was analyzed by using 51 SSR markers. The 111 rice landraces were divided intojaponicagroup (group I) andindicagroup (group II); however, each of the two groups was not com-pletely clustered according to its geographical origin. Most collected landraces, accounting for 83%, belonged toindicagroup. Gelatinization temperature (GT) is an important parameter affecting rice cooking and eating quality. The GT of rice flour was measured, which was widely different among the collected rice varieties, showing four classes in very low ( <66°C), low (66-70°C), high (70-74°C) and very high ( >74°C) GT. Meanwhile,ALK gene, also known asSSSIIa and controlling GT, was sequenced and compared, showing ten haploid types of theALKgene among tested samples. Association analysis, revealed that GT is mainly controlled by the combination of three SNPs ofALK gene. Among them, the A-GC or G-TT combination type con-trols the very low and low GT, while the combination of G-GC controls high and very high GT. SNP3402 type T is associated with high and very high GT. It was also noticed that most of the varieties fromindicagroup tended to contain the G-GC mutation and thus showed a relative high GT compared with those from thejaponicagroup. These results are meaningful for rice molecular breeding and germplasm resources protection.关键词
水稻/ALK基因/遗传多样性/哈尼梯田/糊化温度Key words
Oryza sativa L./ALK gene/Genetic diversity/Hani's terrace/Gelatinization temperature引用本文复制引用
李闯,刘承晨,张昌泉,朱霁晖,徐小颖,赵福伟,黄绍文,金银根,刘巧泉..云南哈尼梯田当前栽培水稻ALK基因的遗传多样性及与稻米糊化温度的关联分析[J].作物学报,2017,43(3):343-353,11.基金项目
本研究由国家重点基础研究发展计划项目(2013CBA01402),国家自然科学基金项目(31561143008,31300324)和江苏省高校自然科学研究面上项目(13KJB180028)资助.This study was supported by the National Basic Research Program of China (2013CBA01402), the National Natural Science Foundation of China (31561143008, 31300324), and the Natural Sciences Research Project of Higher Learning Institution in Jiangsu Province (13KJB180028). （2013CBA01402）
