作物学报2017，Vol.43Issue(3)：354-370,17.DOI:10.3724/SP.J.1006.2017.00354
陆地棉幼苗NaCl胁迫下转录因子的转录组学分析
Transcriptome Analysis of Transcription Factors in Upland Cotton Seedlings under NaCl Stress
摘要
Abstract
A comparative transcriptomics was used to analyze the expression changes of transcription factors and their families in leaves of the two cotton (Gossypium hirsutum L.) varieties (Earlistaple 7 with salt-tolerance and Nandanbadidahua with salt-sensitivity) under 200 mmol L–1 NaCl stress for 4 and 24 hours. We have identified and quantified 2815 transcription factors from 54 transcription factor families of the samples. A total of 249 differentially expressed transcription factor genes of 'Earlista-ple 7' and 261 transcription factor genes of 'Nandanbadidahua' were identified, after 4 hours of NaCl stress. With prolonging stress time (24 hours), the number of differentially expressed transcription factors in two cotton varieties responsive to salt was increased. It was found that the 106 (4 hours) and 184 (24 hours) transcription factor genes were specially expressed in salt-tolerant variety, and the 143 (4 hours) and 282 (24 hours) were exhibited commonly in both cotton lines. We have obtained 124 salt-tolerance-related transcription factor genes from 26 transcription factor families through screening, and further validated the data accuracy by quantitative Real-time PCR. Total 11 highly expressed transcription factors genes by salt induction, including CotAD_66280 (HD-ZIP),CotAD_47058 (ERF),CotAD_18472 (G2-like),CotAD_04289 (C2H2),CotAD_57763 (HSF),Co-tAD_23656 (TCP),CotAD_02221 (ERF),CotAD_23975 (WRKY),CotAD_54036 (MYB),CotAD_27788 (NAC), andCo-tAD_23815 (HSF) were induced to be closely related to salt tolerance in upland cotton, and might be used as a source of candidate tolerant genes for upland cotton breeding.关键词
陆地棉/NaCl胁迫/转录组分析/转录因子Key words
Upland cotton/NaCl stress/Transcriptome analysis/Transcription factor引用本文复制引用
彭振,何守朴,龚文芳,潘兆娥,贾银华,卢艳丽,杜雄明..陆地棉幼苗NaCl胁迫下转录因子的转录组学分析[J].作物学报,2017,43(3):354-370,17.基金项目
本研究由"十二五"国家科技支撑计划项目(2013BAD01B03)和国家自然科学基金项目(31301365)资助. This study was supported by the National Key Technology Support Program of China (2013BAD01B03) and the National Natural Science Foundation of China (31301365). （2013BAD01B03）
