作物学报2017，Vol.43Issue(3)：389-398,10.DOI:10.3724/SP.J.1006.2017.00389
陆地棉微管结合蛋白CLASP家族基因的鉴定及表达分析
Identification and Expression Analysis of Microtubule Binding Protein CLASP Family Genes inGossypium hirsutumL.
摘要
Abstract
CLIP-Associated Proteins (CLASPs) are microtubule associated proteins (MAPS) which are regulators of microtubule structure and function, and play a key role in plant growth and morphogenesis. In this study, sixCLASPs genes were preliminarily identified inGossypiumhirsutumL. by bioinformatics. Based on multiple sequence alignment and phylogenetic tree analysis, we divided the six CLASPs proteins into I and II subfamilies. The subfamily I has HEAT domain and CLASP-N structure; and the subfamily II only has CLASP-N structure. Real-time quantitative reverse transcription PCR (qRT-PCR) revealed thatGhCLASPs genes expressed in root, stem, leaf, petal and fiber at different developmental periods,and there existed different expression pat-terns.CotAD_63740 (GenBank accession No. KX881965) andCotAD_04861 (GenBank accession No. KX881961) were prefer-entially expressed in fiber; CotAD_48232 (GenBank accession No. KX881962), CotAD_48665 (GenBank accession No. KX881963), CotAD_55570 (GenBank accession No. KX881964), and CotAD_68468 (GenBank accession No. KX881966) were preferentially expressed in stem. Different expression patterns suggest that they may play different roles during cotton develop-ment. The results will be helpful for the further analysis of CLASPs proteins in cotton.关键词
陆地棉/微管结合蛋白/微管/表达分析Key words
GossypiumhirsutumL./Microtubule binding protein/Microtubule/Expression analysis引用本文复制引用
朱守鸿,赵兰杰,刘永昌,李艳军,张新宇,孙杰..陆地棉微管结合蛋白CLASP家族基因的鉴定及表达分析[J].作物学报,2017,43(3):389-398,10.基金项目
本研究由国家自然科学基金项目(U1128301),新疆生产建设兵团博士资金专项(2013BB003)和石河子大学高层次人才启动项目(RCZX201218)资助.This study was support by the National Natural Science Foundation of China (U1128301), the Special Research Funds for Doctor of Xinjiang Production and Constraction Crops (2013BB003), and the Startup Project for Outstanding Scholars of Shihezi University (RCZX201218). （U1128301）
