作物学报2017，Vol.43Issue(3)：432-441,10.DOI:10.3724/SP.J.1006.2017.00432
白菜型冬油菜叶片结构和光合特性对冬前低温的响应
Response of Leaf Anatomical Structure and Photosynthetic Characteristics of Winter Turnip Rape (Brassica rapaL.) to Low Temperature before Winter
摘要
Abstract
Stomatal traits, leaf anatomic structure characteristic and diurnal variation of photosynthesis and fluorescence parame-ters, in two winter turnip rape (B. rapaL.) cultivars Longyou 7 (strong cold-tolerant) and Tianyou 4 (weak cold-tolerant) were determined under 0°C and –7.6°C natural low temperature conditions to clearify the effects of low temperature on leaf anatomical structure, mechanism of photosynthesis and cold resistance. With decreasing the temperature before winter, stomatal density, stomatal area, and total stomatal circumference per unit area in two cultivars decreased. Moreover, leaf thickness, palisade tissue and sponge tissue thickness became thinner and cellular space grew bigger. The diurnal variation of net photosynthetic rate of two cultivars presented a single-peak curve, and no typical mid-day depression occurred. Under the lower temperature,Pndeclined with a non-stomatal limitation in two cultivars. Photo inhibition in the two cultivars occurred under low temperature and increased with the temperature decreased. Compared with Longyou 7, Tianyou 4 had bigger stomatal density, stomatal area, total stomatal circumference per unit area and leaf thickness, as well as higher photosynthetic capacity and weaker photo inhibition. In conclu-sion, higher photosynthesis and faster growth in winter turnip rape under lower temperature may result from its bigger stomatal density, stomatal area and total stomatal circumference per unit area as well as thicker leaf, palisade tissue and sponge tissue, which results in a poorer resistance to cold stress. The result may be useful for mining new germplasm and facilitating winter tur-nip rape breeding for cold resistance.关键词
白菜型冬油菜/叶片结构/光合特性/叶绿素荧光参数/低温/抗寒性Key words
Winter turnip rape (Brassica rapa L.)/Leaf anatomical structure/Photosynthesis characteristic/Fluorescence pa-rameters/Low temperature/Cold resistance引用本文复制引用
许耀照,曾秀存,张芬琴,孙佳,孙万仓,武军艳,方彦,刘自刚,孙柏林..白菜型冬油菜叶片结构和光合特性对冬前低温的响应[J].作物学报,2017,43(3):432-441,10.基金项目
本研究由国家自然科学基金项目(31560397, 31460356, 31660401, 31560072),甘肃省自然科学基金项目(145RJZG050, 1506RJZG051)和甘肃省河西走廊特色资源利用重点实验室面上项目(XZ1403)资助.This study was supported by the National Natural Science Foundation of China (31560397, 31460356, 31660401, 31560072), the Gansu Provincial Natural Science Foundation (145RJZG050, 1506RJZG051), and Gansu Province Key Laboratory Program of Hexi Corridor Re-sources Utilization (XZ1403). （31560397, 31460356, 31660401, 31560072）
