作物学报2017，Vol.43Issue(3)：454-463,10.DOI:10.3724/SP.J.1006.2017.00454
低温胁迫下马铃薯的数字基因表达谱分析
Digital Gene Expression Profiling Analysis of Potato under Low Temperature Stress
摘要
Abstract
A variety Cooperation 88 was used to construct cDNA library of potato leaf treated with –2℃ low temperature stress by digital gene expression profiling (DGE) technology, and detect the differentially expressed genes (DEGs). The results of solexa sequencing showed that a total of 28505 DEGs were screened out, 13703 DEGs of which were up-regulated and 14802 DEGs were down-regulated. GO enrichment analysis showed that these DEGs were involved in single-organism metabolic process, oxi-dation-reduction process, metabolisms of carbohydrate, secondary metabolism and catalytic activity. The KEGG enrichment analysis showed that up-regulated genes were significantly associated with phenylpropanoid, photosynthesis-antenna proteins, caro-tenoid biosynthesis, phenylalanine metabolism, starch and sucrose metabolism pathways, while the down-regulated DEGs mainly enriched in plant hormone signal transduction pathway. The differential expressions of four genes were confirmed by Real-time quantitative PCR, showing that the expression changes of these four genes were generally consistent with the results of DGE.关键词
马铃薯/低温胁迫/基因表达谱/差异表达基因Key words
Potato/Low temperature stress/Gene expression profile/Differentially expressed genes引用本文复制引用
杨慧菊,郭华春..低温胁迫下马铃薯的数字基因表达谱分析[J].作物学报,2017,43(3):454-463,10.基金项目
本研究由国家自然科学基金项目(31260342),国家现代农业产业技术体系建设专项(GARS-10)和云南省重大科技专项(2013ZA007)项目资助.This study was supported by the National Natural Science Foundation of China (31260342), the China Agriculture Research System (GARS-10) and Specialized Research Fund for Science and Technology Projects of Yunnan Province (2013ZA007). （31260342）
