中国现代中药2017，Vol.19Issue(1)：11-15,20,6.DOI:10.13313/j.issn.1673-4890.2017.1.002
中华蟾蜍DNA条形码鉴定
Identification of Bufo gargarizans and Its Adulterants by DNA Barcoding Technique
摘要
Abstract
Objective:To study the feasibility of using DNA barcoding technique to identify Bufo gargarizans and its adulterants based on COⅠ gene.Methods:Sequences of partial mitochondrial DNA from cytochrome oxidase subunit Ⅰ (COⅠ)gene of 6 species from Bufo,2 species from Rana,1 species from Pelophylax,and 1 species from Hynobius were downloaded from the GenBank data library.The COⅠ sequences were aligned and edited using ClustalX 1.81 and BioEdit 7.0.9.0 software,respectively.The interspecific and intraspecific genetic distances of different sequences were calculated by Kimura double parameter method using software MEGA 4.0.Based on all the aligned and edited COⅠ sequences,the phylogenetic analyses were conducted using Bayesian inference (BI)and maximum parsimony(MP) to identify B.gargarizans and adulterant.Results:COⅠ sequence haplotypes of different samples of B.gargarizans were gathered together,formed its own monophyly,and distinguished from its adulterants by BI and MP trees.Conclusion:The CoⅠ sequence is ideal DNA barcode to identify B.gargarizans and its adulterants correctly.关键词
中华蟾蜍/细胞色素C氧化酶Ⅰ亚基基因/条形码/鉴定Key words
Bufo gargarizans/COⅠ/DNA barcode/identification引用本文复制引用
赵群,张恬,高波,李灯林,杨艳,李军德..中华蟾蜍DNA条形码鉴定[J].中国现代中药,2017,19(1):11-15,20,6.基金项目
中央本级重大增减支项目(2060302) （2060302）
蟾蜍规范化养殖技术研究(3-YFB2014005) （3-YFB2014005）
安徽省高校省级自然科学研究项目(KJ2016SD61) （KJ2016SD61）
