中国现代中药2017，Vol.19Issue(1)：16-20,5.DOI:10.13313/j.issn.1673-4890.2017.1.003
虻虫的PCR-RFLP鉴别研究
Molecular Identification of Medicinal Gadfly and Its Adulterants by PCR-RFLP Analysis
摘要
Abstract
Objective:To establish a convenient and effective DNA molecular identification method for medicinal gadfly.Methods:CO Ⅰ sequences of gadfly and 7 adulterants were amplified and analyzed,PCR-RFLP primers were designed based on differential fragment,and restriction enzyme digest was further conducted to identify gadfly and the 7 adulterants.Results:Approximately 490 bp specific fragment bands from medicinal gadfly and its adulterants were amplified by MengChong-Dig.F/MengChong-Dig.R.Dra Ⅰ restriction endonuclease could only digest the amplified fragment of medicinal gadfly into two smaller pieces,therefore identifying medicinal gadfly specifically.Conclusion:The methods of PCR-RFLP developed in this research is an accurate identification method for medicinal gadfly.关键词
虻虫/聚合酶链反应-限制性内切酶酶切长度多态性/分子鉴定/COⅠ序列Key words
Medicinal gadfly/PCR-RFLP/molecular authentication/CO Ⅰ sequence引用本文复制引用
蒋超,李军德,袁媛,金艳,赵玉洋..虻虫的PCR-RFLP鉴别研究[J].中国现代中药,2017,19(1):16-20,5.基金项目
中医药行业科研专项(201407003) （201407003）
