中国现代中药2017，Vol.19Issue(1)：21-25,5.DOI:10.13313/j.issn.1673-4890.2017.1.004
快速PCR技术鉴别中药材蛤蚧的方法研究
Molecular Authentication of Gecko and Its Adulterants by Rapid Specific PCR
摘要
Abstract
Objective:To establish a rapid specific PCR method to authenticate gecko and its adulterants.Methods:Specific PCR primers were designed based on CO Ⅰ sequences and the annealing temperature of PCR reaction conditions was optimized,which was performed to authenticate gecko and its 7 adulterants combined with fluorescence analysis.Results:Gecko could obtain a 400 bp specific band in electrophoresis and a great green fluorescence was also observed under 365 nm UV lamp,while the adulterants did not.Conclusion:The results indicated that rapid specific PCR combined with SYBR green Ⅰ fluorescence detection could authenticate gecko and its adulterants rapidly and simply,which provided a new idea and way for gecko rapid identification.关键词
快速PCR/分子鉴定/蛤蚧/荧光检测Key words
Rapid PCR/molecular authentication/gecko/fluorescence detection引用本文复制引用
蒋超,赵群,金艳,袁媛,赵玉洋..快速PCR技术鉴别中药材蛤蚧的方法研究[J].中国现代中药,2017,19(1):21-25,5.基金项目
中医药行业科研专项(201407003) （201407003）
