中国现代中药2017，Vol.19Issue(1)：68-72,5.DOI:10.13313/j.issn.1673-4890.2017.1.014
三种鸡(土从)菌属真菌核苷及嘌呤碱基类成分含量测定
Determination of Nucleoside and Purine Bases in Three Kinds of Fruiting Body of Termitomyces
摘要
Abstract
Objective:To establish an HPLC method for the determination of nucleoside components in the fruiting body of Termitomyces albuminosus,T.eurrhizus and T.aurantiacus.Methods:The assay was performed on a Waters Xbridge C18 (250 mm × 4.6 mm,5 μm) column,mobile phase consisted of methanol-0.1％ orthophosphoric acid water with gradient elution at the flow rate of 0.8 mL·min-1,column temperature was 30 ℃,and the detection wavelength was set at 260 nm.Results:The three kinds of fruiting body of Termitomyces all checked out 2 kinds of purine base and 5 kinds of nucleoside,including hypoxanthine,xanthine,cytidine,adenosine,guanosine,uridine,inosine,and they showed good linearity(r≥0.9996)in the range of 7.51-120.12,4.99-79.84,25.13-402.00,12.61-201.80,5.03-80.48,5.00-80.00,10.00-160.00 μg· mL-1,respectively.The average recoveries (n=6) were within 95.83％-103.01％ with RSD ≤ 3.98％.The contents of 2 kinds of purine base and 5 kinds of nucleoside components in the fruiting body of T.albuminosus were 0.36,3.13,2.20,3.63,1.11,1.87,1.50 mg·g-1,respectively,they in the fruiting body of T.eurrhizus were 0.10,0.78,1.86,3.07,1.48,1.70,0.62 mg· g-1 respectively,and they in the fruiting body of T.aurantiacus were 0.22,2.53,1.86,2.35,0.78,0.84,0.83 mg· g-1,respectively.Conclusion:This HPLC method was simple,accurate and reducible,and can be used for quality evaluation and quality control of Termitomyces.The three kinds of fruiting body of Termitomyces would have higher study value for medicine,nutrition and health food.关键词
鸡(土从)菌属/核苷类/嘌呤碱基/HPLC/含量测定Key words
Termitomyces/nucleoside/purine base/HPLC/content determination引用本文复制引用
高艳云,张园娇,张倩,陈建伟..三种鸡(土从)菌属真菌核苷及嘌呤碱基类成分含量测定[J].中国现代中药,2017,19(1):68-72,5.基金项目
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
我国水生、耐盐中药资源的合理利用研究行业专项(201407002) （201407002）
