安徽农业科学2017，Vol.45Issue(6)：118-120,123,4.
白芷种子蛋白质提取方法的比较
Comparison of Protein Extraction Methods for Angelica dahurica Seeds
摘要
Abstract
[Objective] To establish a suitable protein extraction protocol for total proteins extraction from Angelica dahurica seeds.[Method] We compared the total protein yield and SDS-PAGE electrophoretic band number and strength, lectrophoresis resolution by five different methods.[Result] Method a had higher protein content, less impurity and clearer electrophoresis band.Method b had clearer electrophoresis band, higher resolution, but lower protein content.Methods c and d had more impurity, lower purity and resolution.Method e had higher protein purity, but more lack of protein bands.[Conclusion] Method a has the best extraction effects of protein from A.dahurica seeds, which has higher protein extraction efficiency, less impurity, clearer band, higher resolution, more band number.Thus, method a is superior to other four methods.关键词
白芷种子/蛋白质提取/聚丙烯酰氨凝胶电泳Key words
Angelica dahurica seed/Protein extraction/Polyacrylamide gel electrophoresis分类
农业科技引用本文复制引用
吴萍,郭俊霞,李青苗,夏燕莉,张美,肖特,方清茂..白芷种子蛋白质提取方法的比较[J].安徽农业科学,2017,45(6):118-120,123,4.基金项目
四川省基本科研业务专项(A2015N13) （A2015N13）
四川省科技创新苗子工程(2016077). （2016077）
