安徽农业科学2017，Vol.45Issue(6)：124-127,4.
美洲大蠊蛋白质的提取工艺优化及其对伤口愈合的影响
Optimization of Extraction Process of Periplaneta americana Protein and Its Effect on Wound Healing
摘要
Abstract
[Objective] To optimize extraction method of Periplaneta americana protein and study its effect on wound healing of mice.[Method] The protein was seperated and extracted from P.americana by alkali extraction, salt extraction and enzyme extraction, protein content extracted by different methods were determined, the extraction techniques were optimized;effects of protein from P.americana on wound healing of mice were studied.[Result] The protein yield was highest under three conditions: alkali concentration 1.5%, extraction temperature 80 ℃, extraction time 1.0 h;salt concentration 1.0%, extraction temperature 40 ℃, extraction time 2.0 h;proportion of enzyme 0.6%, enzymolysis temperature 45 ℃, enzymolysis time 2.0 h.Protein from P.americana could promote mice wound healing, and healing time was shortened by nearly half.[Conclusion] Alkali liquor concentration, salt concentration and enzyme concentration have greatest impact on protein extraction.The order of extraction effect is enzyme extraction, alkali extraction, salt extraction, namely the optimal method for extracting protein from P.americana is trypsin extraction.Protein from P.americana can promote mice wound healing.关键词
美洲大蠊/蛋白质/提取工艺/伤口愈合Key words
Periplaneta americana/Protein/Extraction process/Wound healing分类
医药卫生引用本文复制引用
王涛,邹玉,孙小英,王魁源,许龙,罗志文,吕冬云..美洲大蠊蛋白质的提取工艺优化及其对伤口愈合的影响[J].安徽农业科学,2017,45(6):124-127,4.基金项目
佳木斯大学研究生科技创新项目(LZR2015_006). （LZR2015_006）
