安徽医科大学学报2017，Vol.52Issue(3)：369-372,4.DOI:10.19405/j.cnki.issn1000-1492.2017.03.014
bcl-2基因过表达致急性髓系白血病对柔红霉素耐药机制的初步探讨
Over expression of bcl-2 resulting in resistance todaunorubicin in acute myeloid leukemia cells
摘要
Abstract
Objective To explore the relationship between bcl-2 over-expression with the resistance to daunorubicin in acute myeloid leukemia(AML) cell line U937.Methods Construction of pcDNA3.1(+)/bcl-2, and then pcDNA3.1(+)/bcl-2 and pcDNA3.1(+) were transfected into U937 cell line.The cells were devided into 3 groups: U937,U937/NC,U937/bcl-2.These groups were exposed to daunorubicin for 24 h.The growth inhibitory effects of daunorubicin were evaluated by CCK-8.Expression of apoptosis-related proteins was determined byWestern blot.Results Survival rate of cells in U937/ bcl-2 group was markedly higher than cells in U937 and U937/NC groups.The enhanced caspase-3 activity was observed in U937 and U937/NC groups.Conclusion High expression of bcl-2 inhibits activation of caspase-3 and suppression of daunorubicin induced growth inhibitory and generation of drug resistance.关键词
bcl-2/急性髓细胞白血病/凋亡Key words
bcl-2/acute myeloid leukemia/apoptosis分类
医药卫生引用本文复制引用
饶佳,卢玮,王芸芸,周玉兰,张荣艳..bcl-2基因过表达致急性髓系白血病对柔红霉素耐药机制的初步探讨[J].安徽医科大学学报,2017,52(3):369-372,4.基金项目
国家自然科学基金(编号:81200372) （编号:81200372）
江西省卫生计生委科技计划(编号:20165167) （编号:20165167）
