北京林业大学学报2017，Vol.39Issue(3)：81-86,6.DOI:10.13332/j.1000--1522.20160162
蒿柳花粉离体萌发及花粉管生长特性
Characteristics of pollen germination and pollen tube growth in Salix viminalis in vitro
摘要
Abstract
In this study, effects of temperature, light intensity and culture medium on the pollen germination were investigated through in vitro culture in Salix viminalis. The results showed that the pollen germination and pollen tube growth of S. viminalis could be affected by culture temperature and light intensity. The optimum temperature range for pollen germination was 15--25 ℃, and the highest germination rates were obtained under the light intensity of 1500 and 2000 lx. The concentration of sucrose, H3 BO3 and the pH value of culture medium could remarkably promote pollen germination at a certain range based on orthogonal design. The optimal culture condition was:basal medium supplemented with 100 g/L sucrose and 200 mg/L H3 BO3 with a pH value of 5. 0. The rate of pollen germination reached 48. 8% under the conditions of 1000 lx and 25℃. There were no significant differences in the pollen germination between in vitro which used basal medium supplemented with 100 g/L sucrose and 200 mg/L H3 BO3 with a pH value of 5. 0 and pollen germination on stigma.关键词
蒿柳/花粉/离体萌发/萌发率/花粉管Key words
Salix viminalis/pollen/in vitro germination/germination rate/pollen tube分类
农业科技引用本文复制引用
彭向永,刘俊祥,李振坚,程运河,孙振元..蒿柳花粉离体萌发及花粉管生长特性[J].北京林业大学学报,2017,39(3):81-86,6.基金项目
林业公益性行业科研专项(201304115)、中国林业科学研究院基本科研业务费国家重点实验室专项(CAFYBB2016ZX001-3). （201304115）
