北京中医药大学学报2017，Vol.40Issue(2)：119-124,6.DOI:10.3969/j.issn.1006-2157.2017.02.007
消银解毒方颗粒对Jurkat T细胞内JAK1/STAT3信号通路作用机制的研究
Effect of Xiaoyin Jiedu Fangon JAK1/STAT3 signaling pathway in Jurkat T cells
摘要
Abstract
Objective To study the effect of Xiaoyin Jiedu Fang, a Chinese medicine compound formulated by TCM principle of cooling blood with detoxification, on nonreceptor tyrosine kinase JAK1/ signal transducer and activator of transcription 3 (STAT3) signaling pathway in human acute leukemia Jurkat T cells.Methods The abnormal activation of T cells of psoriasis with TCM pattern of blood-heat was induced by stimulating Jurkat T cells with phytohaemagglutinin (PHA) and IL-6.The medicated serum contained Xiaoyin Jiedu Fang granule (XYJDF), Liangxue Fang (LXF) or Jiedu Fang granule (JDF) which was a disassembled prescription of XYJDF, acitretin (positive drug) and distilled water (negative control) were prepared.The model cells were inoculated into 24-hole cell culture plate and were cultured with the prepared medicated serum and Filgotinb(GLPG0634), an inhibitor of JAK/STAT signaling pathway for 48 hours.Jurkat T cells with no treatment was served as the blank group.Protein and mRNA expression of JAK1, STAT3, glucoprotein(GP)130 were detected by using Western blot and real-time PCR.Results The protein and mRNA expression of JAK1,STAT3 and GP130 in the model group were significantly higher than those of the blank group (P<0.01).Compared with the model group, the protein expression of pJAK1/JAK1, pSTAT3/STAT3 and GP130 in all medicated group were decreased significantly (P<0.01), and the mRNA expression of JAK1, STAT3 and GP130 declined (P<0.01 or P<0.05).Conclusion The effect of XYJDF against psoriasis could be via the way of inhibiting JAK1/STAT3 signaling pathway leading to prevent abnormal activation of T cells.关键词
消银解毒方颗粒/人急性T淋巴细胞白血病细胞株/JAK1/STAT3信号通路/大鼠Key words
Xiaoyin Jiedu Fang guanule/Jurkat T cells/JAK1/STAT3 signaling pathway/rats分类
医药卫生引用本文复制引用
陈曦,霍春波,陈广山,张云璧,段行武,张润田,李玲玲..消银解毒方颗粒对Jurkat T细胞内JAK1/STAT3信号通路作用机制的研究[J].北京中医药大学学报,2017,40(2):119-124,6.基金项目
国家自然科学基金面上资助项目(No.81273755),国家自然科学基金青年基金资助项目(No.81403403,No.81403404),北京中医药大学自主选题资助项目(No.2015-JYB-JSMS055),北京中医药大学优青资助项目(No.2016-JYB-XJQ005) （No.81273755）
