北京中医药大学学报2017，Vol.40Issue(2)：125-129,5.DOI:10.3969/j.issn.1006-2157.2017.02.008
活血解毒方对糖尿病大鼠视网膜中Hippo信号通路的影响
Effect of Huoxue Jiedu Recipe on Hippo signaling pathway in diabetic rat retina
摘要
Abstract
Objective To observe the effect of Huoxue Jiedu Recipe(HJR), a Chinese medicine compound, on Hippo signaling pathway in diabetic rat retina.Methods A total 24 healthy SD rats were fasted for 12 h and administered intraperitoneally streptozotocin (65 mg/kg) once to induce diabetic model.After 20 weeks, the diabetic rats were randomly divided into model group and HJR group.And 10 more healthy rats were assigned into normal group with administration intragastrical saline.Each group was intervened once daily for consecutive 12 weeks.The levels of phosphorylated mammalian sterile 20-like kinase(P-MST), large tumor suppressor gene 1 (Lats1), phosphorylated Yes-associated protein(P-YAP), tafazzin (TAZ), TEA domain family member(TEAD) protein in retinal tissue were detected by immunohistochemical method and Western blot.Results Compared with the normal group, the protein expression of P-MST and P-YAP were decreased in the model group.While compared with the model group, the protein expression of P-MST and P-YAP significantly increased in HJR group.And Western blot results showed that protein expression of Lats1, TAZ and TEAD in model group were higher than those of normal group, and HJR decreased the protein expression of Lats1, TAZ and TEAD.Conclusion The effect of Huoxue Jiedu Recipe on diabetic retinopathy may be via regulating Hippo signaling pathway.关键词
活血解毒方/糖尿病视网膜病变/Hippo信号通路/大鼠Key words
Huoxue Jiedu Recipe/diabetic retinopathy/Hippo signaling pathway/rats分类
医药卫生引用本文复制引用
吕甜甜,王宏亮,邢玮,吴晏,王伟,张子剑,韩静..活血解毒方对糖尿病大鼠视网膜中Hippo信号通路的影响[J].北京中医药大学学报,2017,40(2):125-129,5.基金项目
国家自然科学基金资助项目(No.81530100,No.81673705),北京中医药大学自主课题资助项目(No.2016-JYB-XS021) （No.81530100,No.81673705）
