北京中医药大学学报2017，Vol.40Issue(2)：143-147,5.DOI:10.3969/j.issn.1006-2157.2017.02.011
肺纤方提取物对肺间质纤维化大鼠肺微血管VEGF、VEGFR2、PAI-1、VCAM-1mRNA表达的影响
Effect of Feixian Fang extract on mRNA expression of VEGF, VEGFR2, PAI-1 and VCAM-1 in microvascular endothelial cells of rats with pulmonary fibrosis: an in vitro study
摘要
Abstract
Objective To investigate the effect of Feixian Fang extract (FXF) on the expression of vascular endothelial cell growth factor(VEGF), vascular endothelial cell growth factor receptor 2 (VEGFR 2), vascular cell adhesion molecule-1(PAI-1) andplasminogen activator inhibitor-1 (VCAM-1) in pulmonary microvascular endothelial cells in vitro, so as to explore the mechanism of intervention of FXF on microvascular angiogenesis of pulmonary fibrosis.Methods The lung microvascular endothelial cells which were isolated from pulmonary fibrosis model rats were equally divided into model group, losartan group, prednisone group, FXF high-, mid-, and low-dose group.The cells of model group were cultured in DMEM containing 20% fetal bovine serum, those of losartan group were cultured in DMEM containing 20% fetal bovine serum with 10 g/L losartan, those of prednisone group were cultured in DMEM containing 20% fetal bovine serum with 5 mg/L prednisone, and those of FXF groups were cultured in DMEM containing 20% fetal bovine serum with 100, 60, or 20 mg/L Feixian Fang extract.All the cells were cultured for 24 h.Then the mRNA expression of VEGF, VEGFR2, PAI-1 and VCAM-1 were detected by using method of double chain chimeric fluorescent dye SYBR Green real-time fluorescence quantitative detection.Results Campared with the model group, VEGF mRNA expression decreased significantly in all FXF group, while there was no difference of VEGFR2;PAI-1 mRNA was downregulated in FXF high-dose group;VCAM-1 mRNA expression decreased significantly in both FXF high-dose group and FXF low-dose group(P<0.05).Conclusion The effect of FXF against pulmonary fibrosis via the way of improving the blood coagulation and fibrinolysis leading to inhabitation of angiogenesis, due to downregulating the mRNA expression of VEGF, PAI-1 and VCAM-1 in the lung microvascular endothelial cells.关键词
肺纤方提取物/肺间质纤维化微血管内皮细胞/血管内皮细胞生长因子/血管内皮细胞生长因子受体2/纤溶酶原激活物抑制物1/血管细胞黏附因子1/大鼠Key words
Feixian Fang extract/pulmonary interstitial fibrosis microvascular endothelial cells/vascular endothelial cell growth factor/vascular endothelial cell growth factor receptor 2/plasminogen activator inhibitor-1/vascular cell adhesion molecule-1/rats分类
医药卫生引用本文复制引用
王晨,王紫娟,张晓梅,尹婷,刘哲,秦慧慧,张宇婷,顾萧枫,于小林..肺纤方提取物对肺间质纤维化大鼠肺微血管VEGF、VEGFR2、PAI-1、VCAM-1mRNA表达的影响[J].北京中医药大学学报,2017,40(2):143-147,5.基金项目
国家自然科学基金资助项目(No.81273696) （No.81273696）
