北京中医药大学学报2017，Vol.40Issue(2)：159-165,7.DOI:10.3969/j.issn.1006-2157.2017.02.014
基于UPLC-Q-TOF-MS法分析亚香棒虫草化学成分
Study on chemical constituents in Cordyceps hawkesii by UPLC-Q-TOF-MS
摘要
Abstract
Objective To analyze and identify the main chemical constituents of extract from Cordyceps hawkesii by using method of ultra performance liquid chromatography-qudropole time-of-flight mass spectrometry (UPLC-Q-TOF-MS).Methods UPLC condition was as the following: Agilent SB-C18 (50 mm×4.6 mm, 1.8 μm) column with gradient elution consisting of 0.1 % formic acid in water and acetonitrile, at a flow rate of 0.25 mL/min.The column temperature was set at 30℃.MS analysis was based on information associated mode (IDA) that positive and negative ions were respectively collected.Results Mass spectrometry combined with "Formula Finder" of PeakView software and database, a total 26 compounds were identified as alkaloids, glycosides, nucleosides, and amino acids.9 of them had never been reported in Cordyceps hawkesii, and 2 of them were known.Conclusion The method is accurate, reliable and efficient for rapid identification of compounds in Cordyceps hawkesii.The results provide references for the development and utilization of Cordyceps hawkesii, as well as finding out its pharmacodynamic material basis.关键词
亚香棒虫草/超高效液相色谱-四级杆串联飞行时间质谱法/裂解规律/定性分析Key words
Cordyceps hawkes/ultra performance liquid chromatography-qudropole time-of-flight mass spectrometry/cleavage law/qualitative analysis分类
医药卫生引用本文复制引用
秦伟瀚,刘飞,刘翔,张小梅,阳勇..基于UPLC-Q-TOF-MS法分析亚香棒虫草化学成分[J].北京中医药大学学报,2017,40(2):159-165,7.基金项目
国家自然科学基金资助项目(No.30901964、No.81072997),国家中医药行业科技专项资助项目(No.201207002-04) （No.30901964、No.81072997）
