北京中医药大学学报2017，Vol.40Issue(3)：193-199,7.DOI:10.3969/j.issn.1006-2157.2017.03.004
线叶金雀花对小鼠脑缺血再灌注损伤的保护作用
Protective effect of Rooibos on cerebral ischemia-reperfusion injury in mice
摘要
Abstract
ObjectiveTo observe the protective effect of Rooibos (endemic plant in the South Africa) in mice with cerebral ischemia-reperfusion injury (IRI).Methods All mice were divided into control group, IRI group, low-dose Rooibos group (low-dose group, 2.8 g/kg), mid-dose Rooibos group (mid-dose group, 5.6 g/kg) and high-dose Rooibos group (high-dose group, 11.2 g/kg).Before modeling, 3 medicated groups were orally given Rooibos once a day for 7 d, and control group and IRI group were given isometric distilled water.After one week, the model was established by using cerebral middle cerebral artery occlusion (MCAO), and then all groups were continuously given Rooibos or distilled water for 7 d.The influence of Rooibos on cerebral infarction volume was observed after TTC staining.The influence of Rooibos on movement, cognition and sense was observed after neurologic score test, balance beam test, Morris water maze (MWM) test and corner test.The influence of Rooibos on redox state after cerebral injury was observed after detecting the activities of superoxide dismutase (SOD) and glutathione peroxidease (GSH-PX), and content of malondialdehyde (MDA).The influence of Rooibos on inflammatory state after cerebral injury was observed after detecting the brain contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1).Results The cerebral infarction volume decreased significantly (P<0.05), scores of balance beam test decreased significantly (P<0.01), times from corner to non-injury side decreased significantly (P<0.01), and contents of TNF-α, IL-1β and ICAM-1 decreased significantly (P<0.01 or P<0.05) in 3 Rooibos groups compared with IRI group.Conclusion Rooibos has certain protective effect in mice with cerebral IRI, and the effect may be related to the decrease of inflammation factors in brain tissue.关键词
线叶金雀花/脑缺血/神经评分/氧化应激/炎症因子/小鼠Key words
Rooibos/cerebral ischemia/neurologic score/oxidative stress/inflammation factors/mice分类
医药卫生引用本文复制引用
马青,李亚,唐民科..线叶金雀花对小鼠脑缺血再灌注损伤的保护作用[J].北京中医药大学学报,2017,40(3):193-199,7.基金项目
国家科技重大专项"重大新药创制"项目(No.2011ZX09201-201-22),北京中医药大学研究生自主课题项目(No.2016-JYB-XS056) （No.2011ZX09201-201-22）
