北京中医药大学学报2017，Vol.40Issue(3)：241-246,6.DOI:10.3969/j.issn.1006-2157.2017.03.012
针刺联合美多芭对帕金森病小鼠脑多巴胺神经元及蛋白激酶B表达的影响
Influence of acupuncture combining madopar on dopaminergic neurons and Akt expressions in mice with Parkinson's disease
摘要
Abstract
Objective To investigate the protective effect of acupuncture combining madopar on dopaminergic neurons and possible mechanism through observation on the influence of acupuncture in chorea-tremble controlled zone combining madopar on cerebral protein kinase B (Akt) expressions in mice with Parkinson's disease (PD).Methods Male C57BL/6 mice were randomly divided into normal group, model group, madopar group, acupuncture group (A group) and acupuncture combining madopar group (A+M group).The mouse model of PD was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), and madopar group, A group and A+M group were given different therapies.All mice survived for 28 d after modeling.The ethnology of mice was observed by using pole climbing method.The lost of nigra dopaminergic neurons and Akt expressions were detected by using immunohistochemistry technique.Results The time of pole climbing were significantly shorter in madopar group, A group and A+M group than that in model group (P<0.05).After modeling, the expression of nigra dopaminergic neurons decreased in madopar group, A group and A+M group than that in normal group (P<0.05), and increased in A+M group than that in model group and madopar group (P<0.05).The expression of Akt in nigra compacta increased significantly in A+M group than that in other groups (P<0.05).The expressions of Akt at zones of CA1, CA3 and CA4 in hippocampal part increased significantly in A+M group than those in model group and madopar group (P<0.05), and the expressions at zones of CA1 and CA4 increased significantly than those in A group (P<0.05).Conclusion Acupuncture in chorea-tremble controlled zone, especially combining madopar then, can get a therapeutic effect on PD through possibly regulating PI3K/Akt pathway.关键词
帕金森病/1-甲基-4-苯基-1,2,3,6-四氢吡啶/针刺/舞蹈震颤控制区/美多芭/蛋白激酶B/小鼠Key words
Parkinson's disease/1-methyl-4-phenyl-1/2/3/6-tetrahydropyridine/acupuncture/chorea-tremble controlled zone/madopar/protein kinase B/mice分类
医药卫生引用本文复制引用
冯琬迪,郭振宇,王媛媛,盖聪,马浩洁,孙红梅,冯婧,高誉珊,张淑静,许红..针刺联合美多芭对帕金森病小鼠脑多巴胺神经元及蛋白激酶B表达的影响[J].北京中医药大学学报,2017,40(3):241-246,6.基金项目
国家自然科学基金资助项目(No.81573773),北京中医药大学校级自主课题项目(No.2011-JYB22-JS063) （No.81573773）
