北京中医药大学学报2017，Vol.40Issue(3)：247-253,7.DOI:10.3969/j.issn.1006-2157.2017.03.013
电针对控制性超排卵小鼠子宫内膜磷酸化胰岛素样生长因子-1受体表达及其信号转导通路的调控
Regulation effect of electro-acupuncture on expression of endometrium phosphorylation insulin-like growth factor-1 receptor and its signal transduction pathways in mice with controlled ovarian hyperstimulation
摘要
Abstract
Objective To observe the influence of electro-acupuncture (EA) on expression of endometrium phosphorylation insulin-like growth factor-1 receptor (p-IGF-1R), and signal transduction pathways of MAPK and PI3K in mice with controlled ovarian hyperstimulation (COH), and discuss the mechanism of EA improving embryo implantation.Methods Female Kunming mice were randomly divided into natural cycle group, COH group, EA group, EA+BMS-536924 group, EA+LY-294002 group and EA+PD-98059 group (each n=20), and mice were divide again into donor mice and recipient mice.The model was established by applying controlled hyperstimulation program, and given EA in Guanyuan (RN3), Zhongji (RN4) and Sanyinjiao (SP6) at the day of injecting human chorionic gonadotropin (HCG) once a day until stopping collecting samples.The fertilized ovums were collected from donor mice and cultured in vitro, and then transplanted into the uterus of recipient mice.The clinical pregnancy rate and site number of embryo implantation were observed.The expressions of endometrial IGF-1R, p-IGF-1R, phosphorylation protein kinase (p-Akt) and phosphorylation extracellular regulated protein kinases1/2 (p-ERK1/2) were detected by using Western Blot.Results The clinical pregnancy rate, site number of embryo implantation and expressions of endometrial IGF-1R, p-IGF-1R, p-Akt and p-ERK1/2 were all lower in COH group than those in natural cycle group (P<0.05 or P<0.01).All indexes were higher in EA group, EA+LY-294002 group and EA+PD-98059 group than those in COH group (P<0.05 or P<0.01).But the clinical pregnancy rate, site number of embryo implantation had no significant improvement in EA+BMS-536924 group, and expression of p-IGF-1R was significantly lower than that in natural cycle group (P<0.01).The comparison among EA+BMS-536924 group, EA+LY-294002 group and EA+PD-98059 group showed that the expression of p-Akt was higher in EA+PD-98059 group, and expression of p-ERK1/2 was higher in EA+LY-294002 group (P<0.01).Conclusion Embryo implantation can be improved by EA in COH mice, which may be related to EA can up-regulate the expression of p-IGF-1R and signal transduction pathways of PI3K/Akt and MAPK/ERK1/2.关键词
控制性超排卵/电针/磷酸化胰岛素样生长因子-1受体/磷酸化蛋白激激酶B/磷酸化细胞外调节蛋白激酶1/2/小鼠Key words
controlled ovarian hyperstimulation/electro-acupuncture/phosphorylation insulin-like growth factor-1 receptor/phosphorylation protein kinase/phosphorylation extracellular regulated protein kinases1/2/mice分类
医药卫生引用本文复制引用
朱书秀,刘杰,王芳,柳威,李长雷,付蔷,张文娟..电针对控制性超排卵小鼠子宫内膜磷酸化胰岛素样生长因子-1受体表达及其信号转导通路的调控[J].北京中医药大学学报,2017,40(3):247-253,7.基金项目
国家自然科学基金资助项目(No.81373725) （No.81373725）
