草业科学2017，Vol.34Issue(3)：532-538,7.DOI:10.11829/j.issn.1001-0629.2016-0333
白鹤芋染色体制片技术优化及核型分析
Optimization of chromosome sectioning and karyotype analysis of Spathiphyllum floribundum
摘要
Abstract
To establish the optimal approach for analyzing the root tips of Spathiphyllum floribundum‘Mojo’,we compared different sampling times and pretreatments during chromosome sectioning.The results showed that the optimal sampling time was 08:45-09:15 and the optimal pretreatment was 0.002 mol · L-1 or 0.07 mmol · L-1 of 8-hydroxyquinolin or cycloheximide,respectively for 4 h.S.floribundum is a diploid plant and its chromosome number is 2n=2x=30."Mojo" contains a B-chromosome.The karyotype analysis revealed that S.floribundum is 2n=2x=30=20m+6m (SAT) +2sm+2sm(SAT) (B-chromosome).Furthermore,chromosome 3 and 8 are metacentric,whereas the others are facrocentric.Satellites were observed in the four pairs of chromosomes (3,4,6,and 9).The chromosomal length ratio was 1.73,mongolicus was 2A,and the karyotype asymmetry coefficient was 56.37％.关键词
白鹤芋/染色体/制片优化/核型分析Key words
Spathiphyllum floribundum/chromosome sectioning/chromosome/karyotype analysis分类
农业科技引用本文复制引用
张桂芳,任改婷,周佐葡,雷利锋,刘春,张黎..白鹤芋染色体制片技术优化及核型分析[J].草业科学,2017,34(3):532-538,7.基金项目
宁夏科技支撑计划项目《宁夏设施园艺提升发展研究与示范》子课题——小盆花品种资源收集与种苗快繁技术研究与示范(2014ZZN09) （2014ZZN09）
宁夏大学研究生创新项目(GIP201648) （GIP201648）
