长春中医药大学学报2017，Vol.33Issue(2)：181-184,4.DOI:10.13463/j.cnki.cczyy.2017.02.004
电针三阴交对AD小鼠学习记忆能力及海马区Ach、AchE、ChAT的影响
Effects of electroacupuncture at "Sanyinjiao" on learning and memory abilities and brain Ach,AchE and ChAT activities
摘要
Abstract
Objective To observe the effects of electroactupuncture (EA) at "Sanyinjiao" on learning and memory abilities and brain Ach, AchE and ChAT activities in a SAMP8 mouse model of senile dementia.Methods The SAMP8 mice were randomly divided into two groups: model group and EA group. The homologous SAMR1 was used as control group. Each team has six mice. EA group was applied to bilateral "sanyinjiao";Control group and model group were given constraint and no EA treatment. Morris water maze tests were coculated for the SAMP8 mice's learning and memory ability. The hippocampus tissues were collected for detecting the contents of Ach and the activity of AchE and ChAT by ELISA.Results Morris water maze tests results, containing the navigation tests and the space exploration experiments: Compared with the SAMR1 mice, the learning and memory ability of SAMP8 mice were significantly declined (P<0.01). After the treatment with EA, the ability were significantly improved(P<0.05), and the differences was statically signifi cant.Detection index results: compared with the control group, the Ach content and the activity of AchE and ChAT in hippocampus of model group were lower(P<0.01), while after the treatment the level were significantly improved(P<0.05), and the differences was statically significant.Conclusion EA of "Sanyinjiao" can obviously improved the learning and memory ability in AD mice and improved the Ach content in hippocampus. The effect may be achieved by improving the AchE and ChAT activity of hippocampus in AD mice.关键词
老年痴呆/穴,三阴交/学习记忆能力/乙酰胆碱/乙酰胆碱酯酶/乙酰胆碱转移酶Key words
senile dementia/Point/Sanyinjiao(SP6)/learning and memory abilities/acetylcholine/acetylcholinesterase/choline acetyl transferase分类
医药卫生引用本文复制引用
翟丽静,王婧,秦卓,游敏,吴娇娟,李昱颉,程凯..电针三阴交对AD小鼠学习记忆能力及海马区Ach、AchE、ChAT的影响[J].长春中医药大学学报,2017,33(2):181-184,4.基金项目
国家自然科学基金课题"基于NIM网络失衡调节的针灸防治AD的实验研究"(81373726). （81373726）
