长春中医药大学学报2017，Vol.33Issue(2)：185-188,4.DOI:10.13463/j.cnki.cczyy.2017.02.005
平喘固金颗粒对COPD肺脾肾三虚模型大鼠的实验研究
Experimental study on COPD lung spleen and kidney deficiency model rat on Pingchuan Gujin granules
摘要
Abstract
Objective To explore the effects of lung function, lung surface active substance SP-A, IL-8, alveolar lavage Ⅰ , collagen type Ⅲ , right ventricular hypertrophy index and the pathological changes of lung tissue of COPD lung spleen and kidney deficiency model rat on Pingchuan Gujin granules.Methods 60 healthy SD rats were randomly divided to blank control group, model group, the Jinshuibao capsule group,Pingchuan Gujin granules for large, medium and small dose group (10.0, 5.0, 2.5 g/kg), the gas tube drip into the lipopolysaccharide and smoked, and comply with Fanxieye diarrhea methods, back injection of hydrocortisone acetate, to establish lung spleen and kidney deficiency syndrome COPD rats model, the determination of each group in the rat lung function, lung surface active substance SP-A, IL-8, alveolar lavage Ⅰ ,Ⅲ collagen type, right ventricular hypertrophy index, HE dyeing observation lung tissue pathological changes in rats.Results Pingchuan Gujin granules high-dose group of FVC, FEV0.3, FEV0.3 /FVC, PEF increased significantly than the model group (P<0.05); In high-dose group, IL-8 and collagen type Ⅲ content was significantly reduced, higher content of SP-A had no obvious effect on collagen typeⅠ (P<0.05); High-dose group can significantly reduce the right ventricular hypertrophy index of rats (P<0.05); Model group rats bronchial lung HE staining results accord with chronic obstructive pulmonary disease changes, compared with model group, and inflammation absorption good of medium dose group Pingchuan Gujin particles.Conclusion Pingchuan Gujin granules can obviously improve the lung spleen kidney three type virtual COPD rats lung function; Significantly reduce the IL-8 and collagen typeⅢ content; Elevated the SP-A content; Right heart hypertrophy index was significantly reduced; To reduce the lung tissue pathology change.关键词
慢性阻塞性肺疾病/肺脾肾三虚证/SP-A/IL-8/Ⅰ型胶原/右心室肥厚/肺组织病理/大鼠/平喘固金颗粒Key words
COPD/lung spleen and kidney deficiency model/SP-A/IL-8/Ⅰ collagen type/the right ventricle hypertrophy/lung tissue pathology/rats/Pingchuan Gujin granules引用本文复制引用
魏亚东,惠爱武,陈涛,毛家荣,王向阳,苗文红,鱼涛..平喘固金颗粒对COPD肺脾肾三虚模型大鼠的实验研究[J].长春中医药大学学报,2017,33(2):185-188,4.基金项目
陕西省科技计划项目"平喘固金颗粒治疗慢阻肺的实验研究 (2014K11-02-04-01). （2014K11-02-04-01）
