传感技术学报2017，Vol.30Issue(2)：174-183,10.DOI:10.3969/j.issn.1004-1699.2017.02.002
基于RGD多肽掺杂聚吡咯-铟锡氧化物的仿生微电极构建及用于细胞生物学行为的电化学阻抗谱检测
Construction of a Biomimetic Microelectrode Based on RGDPeptide/Polypyrrole-Indium Tin Oxide for Detecting Cell BiologyBehaviors by Electrochemical Impedance Spectroscopy
摘要
Abstract
For fabricating an indium tin oxide(ITO)microelectrode modified with RGD peptide doped polypyrrole(PPy/RGD-ITO)and using it as sensing electrode for detecting cell biology behaviors by electrochemical impedance spectroscopy.The ITO microelectrode was firstly fabricated by etching the insulating layer of photosensitive dry film with lithography technology.Then the PPy/RGD composite was deposited on the surface of the ITO microelectrode by electrochemical copolymerization using the peptide contained RGD motif as the sole dopant to form the PPy/RGD-ITO microelectrode.The surface topology morphology,wettability and composition of the resulted PPy/RGD composite film were characterized by atomic force microscope(AFM),contact angle measurement instrument and fourier transform infrared spectrometer(FTIR)respectively.The human lung cancer A549 cells spreading,adhesion and proliferation experiments were conducted to investigate the interaction between the PPy/RGD composite with cells.Used the PPy/RGD-ITO microelectrode as sensing electrode,A549 cells adhesion and proliferation,as well as the cytotoxicity of Polyphyllin I,a well-known natural anti-cancer molecule,were analyzed by electrochemical impedance spectroscopy.Resulted showed that the RGD molecule was doped into the PPy matrix successfully through the facile electrochemical copolymerization and the resulted PPy/RGD composite film has excellent surface physical properties.The RGD molecule doped into the PPy matrix retained its biology activity and promoted A549 cells spreading,adhesion and proliferation when compared with bare ITO electrode and poly(4-styrene sulfonate)(PSS)doped PPy film.Finally,as the morphological change of A549 cells on the surface of PPy/RGD-ITO microelectrode would change the impedance spectrum characteristics of the electrode system,the information about A549 cells during the process of adhesion and proliferation as well as the quantitative cytotoxicity of Polyphyllin I could be obtained by the electrochemical impedance technology.Therefore,the PPy/RGD film prepared by doping the RGD molecule into the PPy through the facile electrochemical copolymerization showed excellent biocompatibility.In future,the PPy/RGD film could be used as the coupling interface between electronic system and biological cell system and for researching the cellular bio-behaviors and drug screening.关键词
电化学阻抗检测/RGD多肽/聚吡咯/铟锡氧化物/细胞增殖/细胞毒性Key words
electrochemical impedance detection/RGD peptide/polypyrrole/Indium tin oxide/cell proliferation/cytotoxicity分类
信息技术与安全科学引用本文复制引用
李远,肖文海,陈静,廖娟,刘北忠..基于RGD多肽掺杂聚吡咯-铟锡氧化物的仿生微电极构建及用于细胞生物学行为的电化学阻抗谱检测[J].传感技术学报,2017,30(2):174-183,10.基金项目
重庆市自然科学基金面上项目(cstc2012jjA10046) （cstc2012jjA10046）
重庆市卫生局中医药科技项目(ZY20132132) （ZY20132132）
重庆市永川区创新能力建设平台项目(Ycstc2014bf5001) （Ycstc2014bf5001）
重庆市大足区科技计划项目(DZKJ,2014ACC1084) （DZKJ,2014ACC1084）
重庆医科大学附属永川医院院级研究项目(YJZD201302,YJZQN201534) （YJZD201302,YJZQN201534）
